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来自中国西南部的1911年恩格勒和盖尔曼的叶绿体基因组完整组装。

The complete chloroplast genome assembly of Engler and Gehrmann 1911 from southwestern China.

作者信息

Yin Si, Chen Huanhuan, Wu Weijia, Gao Yong

机构信息

College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, China.

出版信息

Mitochondrial DNA B Resour. 2024 May 6;9(5):592-596. doi: 10.1080/23802359.2024.2349771. eCollection 2024.

Abstract

Species in the genus are important cash crops in many tropical and subtropical Asian countries. Although several molecular markers have been employed to determine relationships and assess genetic variation in the genus, some conflicts remain in infrageneric classification and evolution. To aid in the phylogenetic research of the genus, we collected one sample of Engler and Gehrmann 1911 from southwestern China. We assembled the first chloroplast genome of this species using high-throughput sequencing. The assembled genome was 169,341 bp long with a typical quadripartite structure (GenBank accession number: PP234804). The lengths of the large single-copy region, small single-copy region, and two inverted repeats were 90,705 bp, 15,640 bp, 31,498 bp, and 31,498 bp, respectively. We annotated 129 genes across the chloroplast genome of . The phylogenetic trees suggested that the species distributed in continental Asia split into two main clades. The chloroplast genome reported in our study provided valuable genomic resources for the future phylogenetic research of the genus.

摘要

该属的物种在许多亚洲热带和亚热带国家都是重要的经济作物。尽管已经使用了几种分子标记来确定该属内的亲缘关系并评估遗传变异,但在属内分类和进化方面仍存在一些争议。为了辅助该属的系统发育研究,我们从中国西南部采集了一份1911年恩格勒和盖尔曼描述的样本。我们使用高通量测序组装了该物种的首个叶绿体基因组。组装后的基因组长度为169,341 bp,具有典型的四分体结构(GenBank登录号:PP234804)。大单拷贝区域、小单拷贝区域以及两个反向重复序列的长度分别为90,705 bp、15,640 bp、31,498 bp和31,498 bp。我们对该物种叶绿体基因组中的129个基因进行了注释。系统发育树表明,分布在亚洲大陆的该物种分为两个主要分支。我们研究中报道的叶绿体基因组为该属未来的系统发育研究提供了有价值的基因组资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ccd/11075652/59ffc5ec90c1/TMDN_A_2349771_F0001_C.jpg

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