一种用于检测活细胞内源性过氧亚硝酸盐（ONOO）的新型近红外荧光探针。
A Novel Near-Infrared Fluorescent Probe for the Detection of Endogenous Peroxynitrite (ONOO) in Living Cells.
作者信息
Shang Jinting, Gao Wanxia, Cai Junluan, Yang Yan, Wang Chen, Zhao Na, Wang Haiping, Zhang Yibin
机构信息
Huazhong University of Science and Technology, Wuhan, China.
Advanced Technology Institute of Suzhou Chinese Academy of Science, Co., Ltd, Suzhou, China.
出版信息
J Fluoresc. 2025 May;35(5):3091-3098. doi: 10.1007/s10895-024-03733-6. Epub 2024 May 9.
In this study, we present a novel near-infrared (NIR) fluorescent probe Nile-ONO designed for the selective and sensitive detection of ONOO. The probe Nile-ONO employed Nile red as the fluorophore, with diphenylphosphinate serving as the reaction site. In the presence of ONOO, the probe Nile-ONO exhibits remarkable fluorescence enhancement at 659 nm, with a response time of less than 20 min and a low detection limit of 0.32 µM. Importantly, MTT assays demonstrate low cytotoxicity in living cells. Furthermore, Nile-ONO has excellent imaging capabilities for endogenous ONOO. Overall, this work introduces a valuable new method for the rapid detection of ONOO in biological systems.
在本研究中，我们展示了一种新型近红外（NIR）荧光探针尼罗 - ONO，其设计用于选择性和灵敏地检测过氧亚硝酸盐（ONOO⁻）。探针尼罗 - ONO采用尼罗红作为荧光团，二苯基次膦酸酯作为反应位点。在过氧亚硝酸盐存在的情况下，探针尼罗 - ONO在659 nm处表现出显著的荧光增强，响应时间小于20分钟，检测限低至0.32 μM。重要的是，MTT 试验表明其在活细胞中的细胞毒性较低。此外，尼罗 - ONO对内源性过氧亚硝酸盐具有出色的成像能力。总体而言，这项工作为生物系统中过氧亚硝酸盐的快速检测引入了一种有价值的新方法。
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