2021-2022 年济宁市轮状病毒和诺如病毒的流行病学特征及基因特征。
Epidemiological Characteristics and Genotypic Features of Rotavirus and Norovirus in Jining City, 2021-2022.
机构信息
Department of Laboratory, Jining Center for Disease Control and Prevention, Jining 272000, China.
Department of Infectious Disease Control, Shandong Center for Disease Control and Prevention, Jinan 250001, China.
出版信息
Viruses. 2024 Jun 7;16(6):925. doi: 10.3390/v16060925.
Diarrhea, often caused by viruses like rotavirus (RV) and norovirus (NV), is a global health concern. This study focuses on RV and NV in Jining City from 2021 to 2022. Between 2021 and 2022, a total of 1052 diarrhea samples were collected. Real-Time Quantitative Fluorescent Reverse Transcriptase-PCR was used to detect RV-A, NV GI, and NV GII. For RV-A-positive samples, VP7 and VP4 genes were sequenced for genotype analysis, followed by the construction of evolutionary trees. Likewise, for NV-GII-positive samples, VP1 and RdRp genes were sequenced for genotypic analysis, and evolutionary trees were subsequently constructed. Between 2021 and 2022, Jining City showed varying detection ratios: RV-A alone (excluding co-infection of RV-A and NV GII) at 7.03%, NV GI at 0.10%, NV GII alone (excluding co-infection of RV-A and NV GII) at 5.42%, and co-infection of RV-A and NV GII at 1.14%. The highest RV-A ratios were shown in children ≤1 year and 2-5 years. Jining, Jinxiang County, and Liangshan County had notably high RV-A ratios at 24.37% (excluding co-infection of RV-A and NV GII) and 18.33% (excluding co-infection of RV-A and NV GII), respectively. Jining, Qufu, and Weishan had no RV-A positives. Weishan showed the highest NV GII ratios at 35.48% (excluding co-infection of RV-A and NV GII). Genotype analysis showed that, in 2021, G9P[8] and G2P[4] were dominant at 94.44% and 5.56%, respectively. In 2022, G8P[8], G9P[8], and G1P[8] were prominent at 75.86%, 13.79%, and 10.35%, respectively. In 2021, GII.3[P12], GII.4[P16], and GII.4[P31] constituted 71.42%, 14.29%, and 14.29%, respectively. In 2022, GII.3[P12] and GII.4[P16] accounted for 55.00% and 45.00%, respectively. RV-A and NV showed varying patterns for different time frames, age groups, and regions within Jining. Genotypic shifts were also observed in prevalent RV-A and NV GII strains in Jining City from 2021 to 2022. Ongoing monitoring of RV-A and NV is recommended for effective prevention and control.
腹泻，常由轮状病毒（RV）和诺如病毒（NV）等病毒引起，是一个全球性的健康问题。本研究聚焦于 2021 年至 2022 年期间济宁市的 RV 和 NV。2021 年至 2022 年期间，共采集了 1052 份腹泻样本。采用实时荧光定量逆转录聚合酶链反应检测 RV-A、NV GI 和 NV GII。对 RV-A 阳性样本进行 VP7 和 VP4 基因测序进行基因型分析，并构建进化树。同样，对 NV-GII 阳性样本进行 VP1 和 RdRp 基因测序进行基因分型分析，并随后构建进化树。2021 年至 2022 年，济宁市的检测比例存在差异：RV-A 单独（不包括 RV-A 和 NV GII 共同感染）为 7.03%，NV GI 为 0.10%，NV GII 单独（不包括 RV-A 和 NV GII 共同感染）为 5.42%，以及 RV-A 和 NV GII 共同感染为 1.14%。RV-A 比例最高的是≤1 岁和 2-5 岁的儿童。济宁、金乡县和梁山县的 RV-A 比例分别高达 24.37%（不包括 RV-A 和 NV GII 共同感染）和 18.33%（不包括 RV-A 和 NV GII 共同感染）。济宁、曲阜和微山县没有 RV-A 阳性病例。微山县的 NV GII 比例最高，为 35.48%（不包括 RV-A 和 NV GII 共同感染）。基因型分析显示，2021 年 G9P[8]和 G2P[4]占主导地位，分别为 94.44%和 5.56%。2022 年，G8P[8]、G9P[8]和 G1P[8]显著，分别为 75.86%、13.79%和 10.35%。2021 年，GII.3[P12]、GII.4[P16]和 GII.4[P31]分别占 71.42%、14.29%和 14.29%。2022 年，GII.3[P12]和 GII.4[P16]分别占 55.00%和 45.00%。RV-A 和 NV 在济宁市的不同时间段、年龄组和地区表现出不同的模式。在 2021 年至 2022 年期间，济宁市流行的 RV-A 和 NV GII 菌株也发生了基因型变化。建议对 RV-A 和 NV 进行持续监测，以进行有效的预防和控制。
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