人诱导多能干细胞中单核细胞和巨噬细胞的分化方案。
Protocol for differentiation of monocytes and macrophages from human induced pluripotent stem cells.
机构信息
Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20814, USA.
Unit of Clinical and Experimental Immunology, IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine (BioMeTra), University of Milan, 20054 Segrate, Italy.
出版信息
STAR Protoc. 2024 Sep 20;5(3):103217. doi: 10.1016/j.xpro.2024.103217. Epub 2024 Jul 27.
Study of disease-relevant immune cells, namely monocytes and macrophages, is limited based on availability of primary tissue, a limitation that can be remedied using human induced pluripotent stem cell (hiPSC) technology. Here, we present a protocol for differentiation of monocytes and macrophages from hiPSCs. We describe steps for hiPSC maintenance, mesoderm lineage induction, hematopoietic progenitor cells (HPCs) commitment and expansion, and myeloid lineage induction. We then detail procedures for monocyte formation and functional macrophage formation and polarization. For complete details on the use and execution of this protocol, please refer to Chen et al..
研究与疾病相关的免疫细胞，即单核细胞和巨噬细胞，受到原代组织可用性的限制，而这一限制可以通过人类诱导多能干细胞（hiPSC）技术得到弥补。在这里，我们提出了一种从 hiPSC 分化单核细胞和巨噬细胞的方案。我们描述了 hiPSC 维持、中胚层谱系诱导、造血祖细胞（HPC）的诱导和扩增以及骨髓谱系诱导的步骤。然后，我们详细介绍了单核细胞形成和功能性巨噬细胞形成和极化的过程。有关使用和执行本方案的完整详细信息，请参考 Chen 等人的研究。
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