HNRNPA2B1 诱导细胞增殖并作为乳腺癌的生物标志物。
HNRNPA2B1 induces cell proliferation and acts as biomarker in breast cancer.
机构信息
Department of Clinical Laboratory, The Affiliated Changzhou Second People's Hospital, Nanjing Medical University, Changzhou, China.
Department of Blood Transfusion Medicine, School of Medicine, Jinling Hospital, Nanjing University, Nanjing, Jiangsu, China.
出版信息
Cancer Biomark. 2024;40(3-4):285-296. doi: 10.3233/CBM-230576.
BACKGROUND
Numerous studies have shown that m6A plays an important regulatory role in the development of tumors. HNRNPA2B1, one of the m6A RNA methylation reading proteins, has been proven to be elevated in human cancers.
OBJECTIVE
In this study, we aimed to identify the role of HNRNPA2B1 in breast cancer.
METHODS
HNRNPA2B1 expression was investigated via RT-qPCR and TCGA database in breast cancer. Then, the function of HNRNPA2B1 on cancer cell was measured by CCK8 assays, colony formation and scratch assays. In addition, HNRNPA2B1 expression in BRCA was explored via the Wilcoxon signed-rank test, KruskalWallis test and logistic regression. The association with HNRNPA2B1 expression and survival were considered by KaplanMeier and Cox regression analyses. The biological function of HNRNPA2B1 was analyzed via gene set enrichment analysis (GSEA) and the cluster Profiler R software package.
RESULTS
We found that HNRNPA2B1 was highly expressed and induced cell proliferation and migration in breast cancer. Moreover, we observed HNRNPA2B1 induced tumor growth in vivo. In addition, we also found HNRNPA2B1 expression was associated with characteristics and prognosis in breast cancer patients.
CONCLUSION
Our findings suggested that HNRNPA2B1 promoted tumor growth and could function as a new potential molecular marker in breast cancer.
背景
大量研究表明，m6A 在肿瘤的发展中发挥重要的调控作用。HNRNPA2B1 是 m6A RNA 甲基化阅读蛋白之一，已被证明在人类癌症中升高。
目的
本研究旨在确定 HNRNPA2B1 在乳腺癌中的作用。
方法
通过 RT-qPCR 和 TCGA 数据库研究乳腺癌中 HNRNPA2B1 的表达。然后，通过 CCK8 测定、集落形成和划痕测定来测量 HNRNPA2B1 对癌细胞的功能。此外，通过 Wilcoxon 符号秩检验、KruskalWallis 检验和逻辑回归探索 BRCA 中的 HNRNPA2B1 表达。通过 KaplanMeier 和 Cox 回归分析考虑 HNRNPA2B1 表达与生存的关联。通过基因集富集分析（GSEA）和 cluster Profiler R 软件包分析 HNRNPA2B1 的生物学功能。
结果
我们发现 HNRNPA2B1 在乳腺癌中高表达，并诱导细胞增殖和迁移。此外，我们观察到 HNRNPA2B1 在体内诱导肿瘤生长。此外，我们还发现 HNRNPA2B1 表达与乳腺癌患者的特征和预后相关。
结论
我们的研究结果表明，HNRNPA2B1 促进肿瘤生长，可作为乳腺癌的新的潜在分子标志物。
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