中华大刀螳染色体水平基因组组装。

Chromosome-level genome assembly of predatory Arma chinensis.

机构信息

Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

College of Agriculture, Yangtze University, No. 1 Nanhuan Road, Jingzhou, 434025, Hubei, China.

出版信息

Sci Data. 2024 Sep 4;11(1):962. doi: 10.1038/s41597-024-03837-5.

DOI:10.1038/s41597-024-03837-5

PMID:39232013

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11374891/

Abstract

Arma chinensis is a natural enemy that preys on various species and can suppress agricultural and forest pests in the orders Lepidoptera and Coleoptera. Here, we aimed to determine the genome of A. chinensis assembled at the chromosome-level using PacBio and Hi-C technologies. The assembled genome was 986 Mb, with a contig N50 of 2.40 Mb, scaffold N50 of 134.98 Mb, and BUSCO completeness of 96.10%. Hi-C data aided in anchoring the assembly onto seven chromosomes. A sequence of ~ 496.2 Mb was annotated as a repeat element, constituting 51.15% of the genome. We functionally annotated 84.79% of 20,853 predicted protein-encoding genes. This high-quality A. chinensis genome provides a novel genomic resource for future research on Pentatomidae insects.

摘要

中华婪步甲是一种捕食鳞翅目和鞘翅目多种物种的天敌，可抑制农林害虫。在这里，我们旨在使用 PacBio 和 Hi-C 技术组装中华婪步甲的染色体水平基因组。组装的基因组大小为 986Mb，contig N50 为 2.40Mb，scaffold N50 为 134.98Mb，BUSCO 完整性为 96.10%。Hi-C 数据有助于将组装结果锚定到 7 条染色体上。约 496.2Mb 的序列被注释为重复元件，占基因组的 51.15%。我们对 20853 个预测的蛋白质编码基因中的 84.79%进行了功能注释。这个高质量的中华婪步甲基因组为未来研究半翅目昆虫提供了一个新的基因组资源。