探索硒纳米颗粒及制备的硒纳米颗粒@维生素C纳米复合材料减轻尼古丁诱导的大鼠睾丸毒性的潜力。
Exploring the potential of selenium nanoparticles and fabricated selenium nanoparticles @vitamin C nanocomposite in mitigating nicotine-induced testicular toxicity in rats.
作者信息
Hindawy Rabab F, Refaat Rana M M, Fouda Atef E, El-Shishtawy Mohamed A, Kumar Adarsh, El-Shafai Nagi M, Faruk Eman M, Nafea Ola E
机构信息
Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Benha University, Al Nadi Al Ryadi, Qism Benha, Al-Qalyubia Governorate, Benha 13518, Egypt.
Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences (AIIMS), AIIMS Campus, Ansari Nagar East, New Delhi, India.
出版信息
Toxicol Res (Camb). 2024 Sep 30;13(5):tfae154. doi: 10.1093/toxres/tfae154. eCollection 2024 Oct.
BACKGROUND
The tobacco epidemic signifies a major public health threat. Nicotine (NIC), a major active constituent in tobacco, impedes male fertility and semen quality. This work is implemented to explore the potential of selenium nanoparticles (SeNPs) and the newly fabricated SeNPs @vitamin C (SeNPs@VITC) nanocomposite in mitigating testicular toxicity induced by NIC.
MATERIALS AND METHODS
The six groups of 48 adult Wistar rats were designed as follows: the control group injected intraperitoneally with normal saline, the SeNPs group treated orally with 2 mg/kg of SeNPs, the SeNPs@VITC nanocomposite group treated orally with 2 mg/kg of SeNPs@VITC nanocomposite, the NIC group injected intraperitoneally with 1.25 mL/kg of NIC, the NIC+ SeNPs group received SeNPs plus NIC, and the NIC+ SeNPs@VITC nanocomposite group received SeNPs@VITC nanocomposite plus NIC. Treatments were administered over a 28-day period.
RESULTS
NIC treatment significantly caused poor sperm quality, decreased serum testosterone, increased follicle-stimulating hormone (FSH), luteinizing hormone (LH) concentrations, reduced hemoglobin levels, leukocytosis, disrupted testicular oxidant/antioxidant balance, and disorganized testicular structure. The construction of the novel SeNPs@VITC nanocomposite, compared to NIC plus SeNPs alone, demonstrated a more potent ameliorative effect on NIC-induced reproductive toxicity in adult rats. The SeNPs@VITC nanocomposite significantly increased sperm count, reduced the percentage of sperm head abnormalities, lowered both serum FSH and LH concentrations, and improved the hemoglobin response.
CONCLUSIONS
Both SeNPs and SeNPs@VITC nanocomposite alleviated the testicular toxicity induced by NIC, but the SeNPs@VITC nanocomposite exhibited superior efficacy. The SeNPs@VITC nanocomposite could be employed to advance enhanced therapeutic strategies for addressing male infertility.
背景
烟草流行是对公众健康的重大威胁。尼古丁（NIC）是烟草中的主要活性成分，会损害男性生育能力和精液质量。本研究旨在探讨硒纳米颗粒（SeNPs）及新制备的硒纳米颗粒@维生素C（SeNPs@VITC）纳米复合材料减轻尼古丁诱导的睾丸毒性的潜力。
材料与方法
将48只成年Wistar大鼠分为六组，分组如下：对照组腹腔注射生理盐水；SeNPs组口服2mg/kg的SeNPs；SeNPs@VITC纳米复合材料组口服2mg/kg的SeNPs@VITC纳米复合材料；NIC组腹腔注射1.25mL/kg的NIC；NIC + SeNPs组接受SeNPs加NIC；NIC + SeNPs@VITC纳米复合材料组接受SeNPs@VITC纳米复合材料加NIC。处理持续28天。
结果
尼古丁处理显著导致精子质量差、血清睾酮降低、促卵泡激素（FSH）和促黄体生成素（LH）浓度升高、血红蛋白水平降低、白细胞增多、睾丸氧化/抗氧化平衡破坏以及睾丸结构紊乱。与单独的尼古丁加SeNPs相比，新型SeNPs@VITC纳米复合材料对成年大鼠尼古丁诱导的生殖毒性具有更强的改善作用。SeNPs@VITC纳米复合材料显著增加精子数量，降低精子头部异常百分比，降低血清FSH和LH浓度，并改善血红蛋白反应。
结论
SeNPs和SeNPs@VITC纳米复合材料均减轻了尼古丁诱导的睾丸毒性，但SeNPs@VITC纳米复合材料表现出更高的疗效。SeNPs@VITC纳米复合材料可用于推进针对男性不育症的强化治疗策略。
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