中国连续十年呼吸道合胞病毒融合蛋白（F）的累积变异情况
The Cumulative Variations of Respiratory Syncytial Virus Fusion Protein (F) in Ten Consecutive Years in China.
作者信息
Wang Fengjie, Jiang Mingli, Han Zhenzhi, Xu Yanpeng, Sun Yu, Zhu Runan, Chen Dongmei, Guo Qi, Zhou Yutong, Yao Yao, Cao Ling, Qu Dong, Li Muya, Zhao Linqing
机构信息
Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing 100020, China.
Department of Respiratory Medicine, Affiliated Children's Hospital, Capital Institute of Pediatrics, Beijing 100020, China.
出版信息
Infect Dis Rep. 2024 Oct 17;16(5):1017-1029. doi: 10.3390/idr16050081.
BACKGROUND
Variations in the fusion (F) protein of respiratory syncytial virus (RSV) with main antigenic sites I-V and Ø may affect the development of RSV vaccines and therapies.
METHODS
In the study, 30 respiratory specimens positive for RSV were randomly selected from children with acute lower respiratory infections (ALRI) in Beijing every year from 2012 to 2021 for gene sequencing. Then, 300 gene sequences and 508 uploaded to GenBank from China were subjected to phylogenetic analysis.
RESULTS
The results indicated the nucleotide identities were 95.4-100% among 446 sequences of RSV A, and 96.3-100% among 362 of RSV B. The most common variant loci were N80K (100.00%) and R213S (97.76%) for site Ø, and V384I/T (98.43%) for site I among sequences of RSV A, and M152I (100.00%), I185V (100.00%), and L172Q/H (94.48%) for site V, and R202Q (99.45%) for site Ø among sequences of RSV B. N276S appears in 95.29% sequences of RSV A, while S276N and N262 I/S appear in 1.38% and 0.55% sequences of RSV B, respectively. No variation was found in all sequences at the binding sites of 14N4 and motavizumab.
CONCLUSIONS
There were cumulative variations of the RSV gene, especially at some binding sites of antigenic sites.
背景
呼吸道合胞病毒（RSV）融合（F）蛋白中具有主要抗原位点I-V和Ø的变异可能会影响RSV疫苗和治疗方法的研发。
方法
在本研究中，2012年至2021年期间，每年从北京患有急性下呼吸道感染（ALRI）的儿童中随机选取30份RSV阳性呼吸道标本进行基因测序。然后，对300条基因序列以及从中国上传至GenBank的508条序列进行系统发育分析。
结果
结果表明，446条RSV A序列的核苷酸同一性为95.4%-100%，362条RSV B序列的核苷酸同一性为96.3%-100%。在RSV A序列中，位点Ø最常见的变异位点是N80K（100.00%）和R213S（97.76%），位点I是V384I/T（98.43%）；在RSV B序列中，位点V是M152I（100.00%）、I185V（100.00%）和L172Q/H（94.48%），位点Ø是R202Q（99.45%）。N276S出现在95.29%的RSV A序列中，而S276N和N262I/S分别出现在1.38%和0.55%的RSV B序列中。在14N4和莫他维珠单抗的结合位点，所有序列均未发现变异。
结论
RSV基因存在累积变异，尤其是在抗原位点的一些结合位点。
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