铜四胺氟化物对牙釉质抗龋作用的体外研究。
The anti-caries effects of copper tetraamine fluoride on enamel: An in vitro study.
机构信息
Faculty of Dentistry, University of Hong Kong, Hong Kong, China.
Faculty of Dentistry, University of Hong Kong, Hong Kong, China; Department of Mineralized Tissue Biology and Bioengineering, The ADA Forsyth Institute, Cambridge, MA, USA.
出版信息
J Dent. 2024 Dec;151:105446. doi: 10.1016/j.jdent.2024.105446. Epub 2024 Nov 1.
OBJECTIVE
To investigate the antibacterial, remineralising, and discolouring effects of copper tetraamine fluoride (CTF) on artificial enamel caries.
METHOD
Human enamel blocks with artificial caries were treated with CTF, silver diamine fluoride (SDF, positive control) and water (negative control) before being challenged with Streptococcus mutans. The morphology, viability, and growth kinetics of biofilm were evaluated using scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming unit (CFU) counting. The lesion depths, mineral loss, micro-hardness, and crystal characteristics were assessed using micro-computed tomography, Knoop Hardness Tester, and X-ray diffraction (XRD), respectively. The discolouring property was assessed by spectrophotometry.
RESULTS
SEM showed that bacteria completely covered the enamel surface treated with water, but not the enamel surface treated with CTF and SDF. CLSM showed dead-to-live ratio of biofilm treated with CTF, SDF and water were 0.8 ± 0.1, 0.9 ± 0.2 and 0.5 ± 0.1 (p < 0.001, CTF, SDF>Water). Log CFU values of biofilm treated with CTF, SDF and water were 7.7 ± 0.2, 7.7 ± 0.1 and 8.1 ± 0.1 (p < 0.001, CTF, SDF<Water). Lesion-depths (μm) of the CTF, SDF and water groups were 93 ± 6, 92 ± 7 and 152 ± 29 (p < 0.001, CTF, SDF<Water). Mineral loss (gHApcm) of the CTF, SDF and water groups were 0.4 ± 0.1, 0.4 ± 0.2 and 1.0 ± 0.2 (p < 0.001, CTF, SDF<Water). Knoop hardness of the CTF, SDF and water groups were 70 ± 23, 68 ± 16 and 21 ± 10 (p < 0.05, CTF, SDF>Water). XRD revealed well-crystallised hydroxyapatite in enamel treated with CTF and SDF, but not water. Spectrophotometry showed ΔE values of the CTF, SDF and water groups were 5 ± 3, 54 ± 6 and 6 ± 2 (p < 0.001, CTF, water<SDF).
CONCLUSION
CTF inhibited Streptococcus mutans biofilm and remineralised artificial enamel caries without discolouration.
CLINICAL SIGNIFICANCE
If CTF is successfully translated into clinical use, it can be a simple agent for clinicians to arrest enamel caries.
目的
研究铜四胺氟化物（CTF）对人工釉质龋的抗菌、再矿化和变色作用。
方法
用人造龋的牙釉质块分别用 CTF、银胺氟化物（SDF，阳性对照）和水（阴性对照）处理，然后用变异链球菌进行挑战。使用扫描电子显微镜（SEM）、共聚焦激光扫描显微镜（CLSM）和菌落形成单位（CFU）计数评估生物膜的形态、活力和生长动力学。使用微计算机断层扫描、Knoop 硬度测试仪和 X 射线衍射（XRD）分别评估病变深度、矿物质损失、显微硬度和晶体特征。通过分光光度法评估变色性能。
结果
SEM 显示，用清水处理的牙釉质表面完全被细菌覆盖，而用 CTF 和 SDF 处理的牙釉质表面则没有。CLSM 显示，用 CTF、SDF 和水处理的生物膜死菌活菌比为 0.8 ± 0.1、0.9 ± 0.2 和 0.5 ± 0.1（p < 0.001，CTF、SDF>水）。用 CTF、SDF 和水处理的生物膜的 log CFU 值分别为 7.7 ± 0.2、7.7 ± 0.1 和 8.1 ± 0.1（p < 0.001，CTF、SDF<水）。CTF、SDF 和水组的病变深度（μm）分别为 93 ± 6、92 ± 7 和 152 ± 29（p < 0.001，CTF、SDF<水）。CTF、SDF 和水组的矿物质损失（gHApcm）分别为 0.4 ± 0.1、0.4 ± 0.2 和 1.0 ± 0.2（p < 0.001，CTF、SDF<水）。CTF、SDF 和水组的 Knoop 硬度分别为 70 ± 23、68 ± 16 和 21 ± 10（p < 0.05，CTF、SDF>水）。XRD 显示，用 CTF 和 SDF 处理的牙釉质中有结晶良好的羟基磷灰石，但用清水处理的牙釉质中没有。分光光度法显示，CTF、SDF 和水组的ΔE 值分别为 5 ± 3、54 ± 6 和 6 ± 2（p < 0.001，CTF、水<SDF）。
结论
CTF 抑制变形链球菌生物膜并再矿化人工釉质龋，而不会变色。
临床意义
如果 CTF 成功转化为临床应用，它可以成为临床医生阻止釉质龋的简单药物。
Zotero 插件