Polymicrobial interactions influence co-existence and biofilm forming capabilities.

The lungs of patients with cystic fibrosis (CF) are vulnerable to persistent polymicrobial colonization by bacterial pathogens including , and the non-tuberculous mycobacterium (NTM) . The polymicrobial milieu within the CF lung impacts individual species fitness, influences biofilm-forming capabilities, pathogenicity, production of virulence factors and even antimicrobial responses, all potentially compromising therapeutic success. Interaction studies among these CF pathogens are very limited, especially studies on the influences of and on co-existence and virulence. Based on the little known thus far about coinfection of these pathogens, we hypothesize that the co-existence of and alters virulence and phenotypic characteristics. We evaluated the direct (co-culture) and indirect (using supernatant) effects of and on growth rate, biofilm formation, macrophage internalization and glycopeptidolipids (GPL) expression. Our observations indicate that and exert a competitive behavior toward during direct contact or indirect interaction , probably as is the case of polymicrobial infections in the lungs of patients with CF. This is the first report that demonstrates inhibitory effects on growth and biofilm forming capabilities. Collectively, co-culture studies enhance our understanding of polymicrobial interactions during coinfection and can guide to establish better management of coinfections and treatment strategies for .