Differential gene expression profile in treated human gingival keratinocytes and their role in the development of HNSCC.

BACKGROUND

Periodontitis is considered to be one of the major risk factors associated with cancers of the oral cavity. Periodontogenic pathogens such as and are the important pathogens associated with periodontitis. Chronic exposure to bacterial components induces changes in the nearby cells. Hence, the present study has been designed to identify the molecular mechanisms that could be associated with the two disease conditions periodontitis and head and neck cancer.

OBJECTIVE

The present study investigated the differential gene expression profile in human gingival keratinocytes treated with a bacterium associated with periodontal disease, and its possible association with the development of Head and Neck Squamous Cell Carcinoma (HNSCC).

METHODS

The study followed a computational design. Multiple tools and databases, such as GEOmnibus, STRING, Metascape, PANTHER, and UALCAN, cBioportal, were used to derive an association between gene expression during infection with and the resulting gene expression profiles were analyzed in the HNSCC dataset.

RESULTS

The study revealed 29 genes from a pool of transcripts acquired after comparing the Pg-HIGK and Sham-HIGK. Among them, 3 genes i.e., and were found to be overexpressed and significantly influenced patient survival. The upregulation of was found to correlate with poor prognosis in HNSCC patients.

CONCLUSION

The study provided insights into the possible association of and in the development of HNSCC. Further investigations are warranted to confirm the functional role of these genes in establishing the cancer phenotype in patients with chronic infection with .