López-Lira Claudia, Valencia Pedro, Urtubia Alejandra, Landaeta Esteban, Tapia Ricardo A, Franco Wendy
Departamento de Química y Bioprocesos, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, Santiago 7820244, Chile.
Centro de Investigación Daniel Alkalay Lowitt, Universidad Técnico Federico Santa María, Av. España 1680, Valparaíso 2390123, Chile.
Foods. 2024 Dec 23;13(24):4166. doi: 10.3390/foods13244166.
In this study, the influences of inorganic nitrogen source (INS) and organic nitrogen source (ONS) supplementation during the wine fermentation process using three non-Saccharomyces yeasts (, , and ) were analyzed. Diamine phosphate (DAP) was used as an INS, and lees enzymatic hydrolysate was used as an ONS. Complete alcoholic fermentation and a higher concentration of volatile compounds were obtained in fermentations with ONS, mainly esters from 81 to 4564 µg/L, alcohols from 231 to 7294 µg/L, and isoamyl acetate ester compounds from 12.3-22.8 ppb, with a very marked odorant activity value (OAV). In addition, malic acid was detected due to its influence on yeast metabolism and, consequently, on aroma production. Using a Y15 enzymatic autoanalyzer, residues of 1.30 g/L in ONS and 1.35 g/L in INS were obtained on the last day of alcoholic fermentation. In summary, we obtained promising results concerning the production of wine with enhanced functionalities due to higher concentrations of some volatile and polyphenolic compounds.
在本研究中,分析了在使用三种非酿酒酵母(、和)进行葡萄酒发酵过程中添加无机氮源(INS)和有机氮源(ONS)的影响。磷酸二胺(DAP)用作INS,酒糟酶解产物用作ONS。在使用ONS的发酵中实现了完全酒精发酵,并获得了更高浓度的挥发性化合物,主要是81至4564μg/L的酯类、231至7294μg/L的醇类以及12.3 - 22.8 ppb的乙酸异戊酯类化合物,具有非常显著的气味活性值(OAV)。此外,检测到了苹果酸,因为它对酵母代谢有影响,进而对香气产生有影响。在酒精发酵的最后一天,使用Y15酶自动分析仪,在ONS中获得了1.30 g/L的残留量,在INS中获得了1.35 g/L的残留量。总之,由于某些挥发性和多酚类化合物浓度较高,我们在生产具有增强功能的葡萄酒方面取得了有前景的结果。