Negative Selection in × Hybrids Indicates Incompatible Oxidative Phosphorylation (OXPHOS) Proteins.

Crossing () females with () males results in all-male progeny that are essential for effective tilapia aquaculture. However, a reproductive barrier between these species prevents commercial-scale yield. To achieve all-male progeny, the currently used practice is crossing admixed stocks and feeding fry with synthetic androgens. Hybrid tilapias escaping to the wild might impact natural populations. Hybrids competing with wild populations undergo selection for different stressors, e.g., oxygen levels, salinity, and low-temperature tolerance. Forming mitochondrial oxidative phosphorylation (OXPHOS) complexes, mitochondrial (mtDNA) and nuclear DNA (nDNA)-encoded proteins control energy production. Crossbred tilapia have been recorded over 60 years, providing an excellent model for assessing incompatibility between OXPHOS proteins, which are critical for the adaptation of these hybrids. Here, by comparing nonconserved amino acid substitutions, across 116 OXPHOS proteins, between and , we developed a panel of 13 species-specific probes. Screening 162 SRA experiments, we noted that 39.5% had a hybrid origin with mtDNA-nDNA allele mismatches. Observing that the frequency of interspecific mtDNA-nDNA allele combinations was significantly ( < 10) lower than expected for three factors, , , and , we concluded that these findings likely indicated negative selection, cytonuclear incompatibility, and a reproductive barrier.