用于检测与脑炎相关病毒的多重实时聚合酶链反应的诊断准确性：一项系统评价和Meta分析方案
Diagnostic accuracy of multiplex real-time PCR for detecting viruses associated with encephalitis: A systematic review and meta-analysis protocol.
作者信息
Syed Hussin Sharifah Aliah Diyanah, Chen Xin Wee, Al-Talib Hassanain, Chua Ang-Lim, Azimi Ziauddin, Wang Seok Mui
机构信息
Institute of Medical Molecular Biotechnology (IMMB), Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia.
Department of Public Health Medicine, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia.
出版信息
PLoS One. 2025 Mar 13;20(3):e0318805. doi: 10.1371/journal.pone.0318805. eCollection 2025.
BACKGROUND
Encephalitis is the most common infectious disease of the central nervous system and is associated with high morbidity, mortality, and disability. Therefore, rapid and accurate diagnosis is crucial to provide patients with timely and appropriate therapeutic intervention. In this study, a comprehensive systematic review with meta-analysis will be conducted to summarize the available data and evaluate the diagnostic accuracy of multiplex real-time polymerase chain reaction (PCR) in the detection of viral encephalitis.
METHODS
We will search PubMed, MEDLINE, EMBASE, Web of Science (WoS), Scopus and Cochrane Library databases for studies evaluating the diagnostic accuracy of multiplex PCR for the diagnosis of encephalitis caused by viruses from January 2014 to December 2024. Observational study designs with full text will be exported and included. Risk of bias will be assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. Analyses will be performed using the "mada" package of R software (R Foundation for Statistical Computing, Vienna, Austria), and the Summary Receiver Operating Characteristic (SROC) will be calculated using the "midas" package of STATA version 15.0 (Stata Corp., College Station, TX, USA). Certainty of evidence will be performed using Grading of Recommendations Assessment, Development and Evaluation (GRADE) software.
RESULTS
The results will provide clinical evidence for the diagnostic accuracy of the multiplex PCR assay for the detection of viruses that cause encephalitis, including sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (LR+), and negative likelihood ratio (LR-). Finally, we intent to submit this systematic review and meta-analysis to a peer-reviewed journal for publication.
CONCLUSION
This systematic review aims to provide current evidence for multiplex PCR assay for the diagnosis of viruses causing encephalitis. Importantly, this study focuses on the use of multiplex PCR for viral diagnosis and helps clinicians and patients to better understand its role in the diagnosis of CNS diseases.
SYSTEMATIC REVIEW REGISTRATION
PROSPERO registration number: CRD42023485942.
背景
脑炎是中枢神经系统最常见的传染病，与高发病率、死亡率和残疾率相关。因此，快速准确的诊断对于为患者提供及时适当的治疗干预至关重要。在本研究中，将进行一项综合系统评价并进行荟萃分析，以总结现有数据并评估多重实时聚合酶链反应（PCR）在检测病毒性脑炎中的诊断准确性。
方法
我们将在PubMed、MEDLINE、EMBASE、科学网（WoS）、Scopus和Cochrane图书馆数据库中检索2014年1月至2024年12月期间评估多重PCR诊断病毒引起的脑炎的诊断准确性的研究。将导出并纳入具有全文的观察性研究设计。将使用诊断准确性研究质量评估（QUADAS-2）工具评估偏倚风险。将使用R软件的“mada”包（奥地利维也纳的R统计计算基金会）进行分析，并将使用STATA 15.0版本的“midas”包（美国德克萨斯州大学站的Stata公司）计算汇总接受者操作特征（SROC）。将使用推荐分级评估、制定和评价（GRADE）软件确定证据的确定性。
结果
结果将为多重PCR检测导致脑炎的病毒的诊断准确性提供临床证据，包括敏感性、特异性、阳性预测值（PPV）、阴性预测值（NPV）、阳性似然比（LR+）和阴性似然比（LR-）。最后，我们打算将这一系统评价和荟萃分析提交给同行评审期刊发表。
结论
本系统评价旨在为多重PCR检测诊断引起脑炎的病毒提供当前证据。重要的是，本研究侧重于多重PCR在病毒诊断中的应用，有助于临床医生和患者更好地理解其在中枢神经系统疾病诊断中的作用。
系统评价注册
PROSPERO注册号：CRD42023485942。
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