从泗水一个传统市场的鸡蛋壳拭子中分离出的多重耐药菌中blaSHV基因的分子检测。

Molecular detection of blaSHV gene in multidrug resistance of isolated from chicken egg shell swab from a traditional market in Surabaya.

作者信息

Sulaiman Azhari Athaillah, Effendi Mustofa Helmi, Srimaryanto Leonardo Reza, Khairullah Aswin Rafif, Kurniasari Pitaloka, Tyasningsih Wiwiek, Moses Ikechukwu Benjamin, Ahmad Riza Zainuddin, Rehman Saifur, Budiastuti Budiastuti, Afnani Daniah Ashri, Yanestria Sheila Marty, Riwu Katty Hendriana Priscilia, Widodo Agus

机构信息

Master Program in Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.

Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.

出版信息

Open Vet J. 2025 May;15(5):2193-2205. doi: 10.5455/OVJ.2025.v15.i5.37. Epub 2025 May 31.

DOI:10.5455/OVJ.2025.v15.i5.37

PMID:40557075

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12184469/

Abstract

BACKGROUND

Contamination with food ingredients, including eggs, causes various dangers because it threatens public health, because it acts as a multidrug resistance (MDR) bacteria, especially the extended-spectrum beta-lactamase (ESBL) strain. The ESBL SHV gene is part of a broad-spectrum ESBL that is often found in Gram-negative bacteria.

AIM

This study aimed to identify the ESBL SHV gene in MDR from chicken eggshells.

METHODS

This study used 160 samples of chicken eggshell swabs isolated on 1% BPW media from 10 traditional Surabaya markets. Samples were isolated using MCA media and were identified using Gram staining and biochemical tests. Detection of MDR using Muller-Hinton Agar.

RESULTS

Confirmation of ESBL in multidrug-resistant (MDR) isolates was performed using polymerase chain reaction to detect ESBL genes. The results showed that the isolation and identification of bacteria were 25.62% (41/160). Amoxicillin antibiotics showed the highest level of resistance at a percentage of 100% (41/41), followed by antibiotic resistance to erythromycin (90.24% (37/41), Streptomycin antibiotics were 26.82% (11/41), ciprofloxacin (14.63% (6/41), and Tetracycline antibiotic resistance was 7.31% (3/41). The results of MDR from showed 34.14% (14/41) of the isolates were then tested by PCR, which showed positive results for the SHV gene of 71.42% (10/14).

CONCLUSION

The data from this study confirm the existence of bacteria isolated from egg shell swabs carrying the SHV gene from MDR isolates.

摘要

背景

包括鸡蛋在内的食品成分污染会引发各种危险，因为它威胁公众健康，还因为它是一种多重耐药（MDR）细菌，尤其是超广谱β-内酰胺酶（ESBL）菌株。ESBL SHV基因是广谱ESBL的一部分，常见于革兰氏阴性菌中。

目的

本研究旨在鉴定来自鸡蛋壳的多重耐药菌中的ESBL SHV基因。

方法

本研究使用了从泗水10个传统市场采集的160份鸡蛋壳拭子样本，在1% BPW培养基上进行分离。样本使用MCA培养基进行分离，并通过革兰氏染色和生化试验进行鉴定。使用Muller-Hinton琼脂检测多重耐药性。

结果

使用聚合酶链反应检测ESBL基因，对多重耐药（MDR）分离株中的ESBL进行确认。结果显示，细菌的分离和鉴定率为25.62%（41/160）。阿莫西林抗生素的耐药水平最高，为100%（41/41），其次是对红霉素的耐药率为90.24%（37/41），链霉素抗生素为26.82%（11/41），环丙沙星为14.63%（6/41），四环素抗生素耐药率为7.31%（3/41）。然后对34.14%（14/41）的分离株进行多重耐药性检测，通过PCR检测，结果显示SHV基因阳性率为71.42%（10/14）。