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不止于生死:3D生物打印组织成像与分析的进展

More than just life and death: advances in imaging and analysis for 3D-bioprinted tissues.

作者信息

Spiller Erin R, Duarte Campos Daniela F

机构信息

Bioprinting & Tissue Engineering Group, Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany.

出版信息

Front Bioeng Biotechnol. 2025 Jun 11;13:1600077. doi: 10.3389/fbioe.2025.1600077. eCollection 2025.

Abstract

3D bioprinting is a fast-growing field with applications in both microphysiological systems and tissue engineering. However, the qualifications and definitions of success for 3D-bioprinted products are insufficient. We can further our characterization of 3D-bioprinting methods and finished products using new imaging techniques and analysis methods, including the use of AI tools. This multi-faceted approach can deepen our understanding of valuable technology by examining the effects of 3D bioprinting on cell identity, behavior and organelles. Defining a successful 3D-bioprinted product in addition to viability is crucial in the push toward using these models for drug screening or disease modeling, where robust and high-quality systems are required for meaningful data output.

摘要

3D生物打印是一个快速发展的领域,在微生理系统和组织工程中都有应用。然而,3D生物打印产品成功的标准和定义并不充分。我们可以使用新的成像技术和分析方法,包括人工智能工具的使用,进一步对3D生物打印方法和成品进行表征。这种多方面的方法可以通过研究3D生物打印对细胞特性、行为和细胞器的影响,加深我们对这项有价值技术的理解。除了细胞活力之外,定义一个成功的3D生物打印产品对于推动将这些模型用于药物筛选或疾病建模至关重要,因为在这些应用中需要强大且高质量的系统才能产生有意义的数据输出。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79f5/12187805/68ef7dc4ceb2/fbioe-13-1600077-g001.jpg

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