用于基因工程动物定制的CRISPR-Cas9技术

CRISPR-Cas9 in the Tailoring of Genetically Engineered Animals.

作者信息

Urban Wiktoria, Kropacz Marta, Łach Maksymilian, Jankowska Anna

机构信息

Department of Cell Biology, Poznan University of Medical Sciences, 61-701 Poznań, Poland.

出版信息

Curr Issues Mol Biol. 2025 May 4;47(5):330. doi: 10.3390/cimb47050330.

DOI:10.3390/cimb47050330

PMID:40699729

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12109904/

Abstract

CRISPR-Cas9 enables targeted genome editing and has become a pivotal tool in biomedical research and animal genome engineering. This review highlights its application in generating genetically modified animals used as preclinical disease models, bioreactors for recombinant protein production, and potential sources of xenotransplantation organs. We also discuss its role in improving livestock traits, welfare, and breeding efficiency. The benefits and limitations of CRISPR-Cas9 are examined, emphasizing its transformative potential in research and agricultural biotechnology.

摘要

CRISPR-Cas9技术可实现靶向基因组编辑，已成为生物医学研究和动物基因组工程中的关键工具。本综述重点介绍了其在生成用作临床前疾病模型的转基因动物、用于重组蛋白生产的生物反应器以及异种移植器官潜在来源方面的应用。我们还讨论了其在改善家畜性状、福利和繁殖效率方面的作用。本文审视了CRISPR-Cas9的优势与局限性，强调了其在研究和农业生物技术中的变革潜力。