苜蓿根瘤菌赖氨酰 - tRNA合成酶和谷氨酰胺 - tRNA合成酶基因的共转录

Co-transcription of Rhizobium meliloti lysyl-tRNA synthetase and glutamyl-tRNA synthetase genes.

作者信息

Pelchat M, Gagnon Y, Laberge S, Lapointe J

机构信息

Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Sainte-Foy, Que., Canada.

出版信息

FEBS Lett. 1999 Apr 16;449(1):23-7. doi: 10.1016/s0014-5793(99)00385-3.

DOI:10.1016/s0014-5793(99)00385-3

PMID:10225420

Abstract

An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypeptide of the expected mass (62 kDa), thus confirming that it encodes R. meliloti lysyl-tRNA synthetase. Reverse transcription/polymerase chain reaction was used to demonstrate that this lysS gene is co-transcribed with gltX in R. meliloti. This is the first reported case of two immediately adjacent and co-transcribed genes encoding aminoacyl-tRNA synthetases.

摘要

在编码苜蓿中华根瘤菌谷氨酰胺-tRNA合成酶的gltX下游10个核苷酸处，发现了一个开放阅读框，其编码的假定多肽与几种赖氨酰-tRNA合成酶非常相似。该基因的表达弥补了大肠杆菌lysS基因的突变，并导致预期分子量（62 kDa）的多肽过度表达，从而证实它编码苜蓿中华根瘤菌赖氨酰-tRNA合成酶。采用逆转录/聚合酶链反应证明，该lysS基因在苜蓿中华根瘤菌中与gltX共转录。这是首次报道的两个紧邻且共转录的编码氨酰-tRNA合成酶的基因。