铜绿假单胞菌谷氨酰胺-tRNA合成酶（gltX）基因的克隆
Cloning of the glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa.
作者信息
Franklund C V, Goldberg J B
机构信息
Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA.
出版信息
J Bacteriol. 1999 Jun;181(11):3582-6. doi: 10.1128/JB.181.11.3582-3586.1999.
Abstract
The glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa was identified. A plasmid containing a 2.3-kb insert complemented the temperature-sensitive gltX mutation of Escherichia coli JP1449, and GltX activity was demonstrated. The inferred amino acid sequence of this gene showed 50.6% identity with GltX from Rhizobium meliloti.
摘要
鉴定出了铜绿假单胞菌的谷氨酰胺-tRNA合成酶（gltX）基因。一个含有2.3 kb插入片段的质粒互补了大肠杆菌JP1449的温度敏感型gltX突变，并证明了GltX活性。该基因推导的氨基酸序列与苜蓿根瘤菌的GltX有50.6%的同一性。
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本文引用的文献
1
Evolutionary anomalies among the aminoacyl-tRNA synthetases.
Curr Opin Genet Dev. 1998 Dec;8(6):630-6. doi: 10.1016/s0959-437x(98)80030-0.
2
Aminoacyl tRNA synthetases as targets for new anti-infectives.
FASEB J. 1998 Dec;12(15):1599-609.
3
Modular evolution of the Glx-tRNA synthetase family--rooting of the evolutionary tree between the bacteria and archaea/eukarya branches.
Eur J Biochem. 1998 Aug 15;256(1):80-7. doi: 10.1046/j.1432-1327.1998.2560080.x.
4
Overproduction of the Bacillus subtilis glutamyl-tRNA synthetase in its host and its toxicity to Escherichia coli.
Can J Microbiol. 1998 Apr;44(4):378-81.
5
Cloning and characterization of the gene (rfc) encoding O-antigen polymerase of Pseudomonas aeruginosa PAO1.
Gene. 1995 Dec 29;167(1-2):81-6. doi: 10.1016/0378-1119(95)00595-1.
6
The glutamyl-tRNA synthetase of Escherichia coli contains one atom of zinc essential for its native conformation and its catalytic activity.
Biochemistry. 1993 Oct 26;32(42):11390-6. doi: 10.1021/bi00093a016.
7
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J Biol Chem. 1995 Jun 23;270(25):15162-9. doi: 10.1074/jbc.270.25.15162.
8
Architectures of class-defining and specific domains of glutamyl-tRNA synthetase.
Science. 1995 Mar 31;267(5206):1958-65. doi: 10.1126/science.7701318.
9
The rfb locus from Pseudomonas aeruginosa strain PA103 promotes the expression of O antigen by both LPS-rough and LPS-smooth isolates from cystic fibrosis patients.
Mol Microbiol. 1994 Aug;13(3):427-34. doi: 10.1111/j.1365-2958.1994.tb00437.x.
10
The Pseudomonas aeruginosa algC gene encodes phosphoglucomutase, required for the synthesis of a complete lipopolysaccharide core.
J Bacteriol. 1994 Jun;176(12):3500-7. doi: 10.1128/jb.176.12.3500-3507.1994.
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