秀丽隐杆线虫固有免疫中一条保守的p38丝裂原活化蛋白激酶信号通路。

A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity.

作者信息

Kim Dennis H, Feinbaum Rhonda, Alloing Geneviève, Emerson Fred E, Garsin Danielle A, Inoue Hideki, Tanaka-Hino Miho, Hisamoto Naoki, Matsumoto Kunihiro, Tan Man-Wah, Ausubel Frederick M

机构信息

Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.

出版信息

Science. 2002 Jul 26;297(5581):623-6. doi: 10.1126/science.1073759.

DOI:10.1126/science.1073759

PMID:12142542

Abstract

A genetic screen for Caenorhabditis elegans mutants with enhanced susceptibility to killing by Pseudomonas aeruginosa led to the identification of two genes required for pathogen resistance: sek-1, which encodes a mitogen-activated protein (MAP) kinase kinase, and nsy-1, which encodes a MAP kinase kinase kinase. RNA interference assays and biochemical analysis established that a p38 ortholog, pmk-1, functions as the downstream MAP kinase required for pathogen defense. These data suggest that this MAP kinase signaling cassette represents an ancient feature of innate immune responses in evolutionarily diverse species.

摘要

一项针对秀丽隐杆线虫突变体的基因筛选，这些突变体对铜绿假单胞菌的杀伤作用敏感性增强，结果鉴定出了两个病原体抗性所需的基因：sek-1，它编码一种丝裂原活化蛋白（MAP）激酶激酶；以及nsy-1，它编码一种MAP激酶激酶激酶。RNA干扰试验和生化分析表明，一个p38直系同源物pmk-1作为病原体防御所需的下游MAP激酶发挥作用。这些数据表明，这个MAP激酶信号转导盒代表了进化上不同物种先天免疫反应的一个古老特征。