通过实时聚合酶链反应（PCR）在有呼吸道症状患者的样本中，呼吸道合胞病毒、流感病毒、副流感病毒和腺病毒的检测率增加。
Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms.
作者信息
van de Pol Alma C, van Loon Anton M, Wolfs Tom F W, Jansen Nicolaas J G, Nijhuis Monique, Breteler Els Klein, Schuurman Rob, Rossen John W A
机构信息
Department of Virology, Eijkman-Winkler Center, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
出版信息
J Clin Microbiol. 2007 Jul;45(7):2260-2. doi: 10.1128/JCM.00848-07. Epub 2007 May 16.
Abstract
Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.
摘要
对有呼吸道症状的住院患者的呼吸道样本（n = 267）进行了实时聚合酶链反应（PCR）、病毒培养以及呼吸道合胞病毒、流感病毒、副流感病毒和腺病毒的直接免疫荧光检测。与传统诊断测试相比，实时PCR将儿童和成人这些病毒的诊断率分别从24%提高到43%，从3.5%提高到36%。
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