用于从临床肠热症患者血液中分离出的沙门氏菌中鉴定伤寒沙门氏菌、甲型副伤寒沙门氏菌和乙型副伤寒沙门氏菌血清型的聚合酶链反应方法。
PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever.
作者信息
Levy Haim, Diallo Souleymane, Tennant Sharon M, Livio Sofie, Sow Samba O, Tapia Milagritos, Fields Patricia I, Mikoleit Matthew, Tamboura Boubou, Kotloff Karen L, Lagos Rosanna, Nataro James P, Galen James E, Levine Myron M
机构信息
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
出版信息
J Clin Microbiol. 2008 May;46(5):1861-6. doi: 10.1128/JCM.00109-08. Epub 2008 Mar 26.
Abstract
PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.
摘要
聚合酶链反应（PCR）方法被开发用于鉴定伤寒沙门氏菌、甲型副伤寒沙门氏菌和乙型副伤寒沙门氏菌血清型。一种多重PCR可鉴定D群、A群和B群以及Vi阳性菌株；另一种则可确认鞭毛抗原“d”、“a”或“b”。对664份马里和智利沙门氏菌血液分离株进行的盲法检测显示，其灵敏度和特异性均为100%。
相似文献
1
PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever.
J Clin Microbiol. 2008 May;46(5):1861-6. doi: 10.1128/JCM.00109-08. Epub 2008 Mar 26.
2
Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A.
J Clin Microbiol. 2002 Feb;40(2):633-6. doi: 10.1128/JCM.40.02.633-636.2002.
3
Antimicrobial susceptibility pattern and sequence analysis of DNA gyrase and DNA topoisomerase IV in Salmonella enterica serovars Typhi and Paratyphi A isolates with decreased susceptibility to ciprofloxacin.
Trans R Soc Trop Med Hyg. 2016 Aug;110(8):472-9. doi: 10.1093/trstmh/trw051. Epub 2016 Sep 12.
4
Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samples.
Epidemiol Infect. 2009 Jan;137(1):102-7. doi: 10.1017/S0950268808000654. Epub 2008 Apr 16.
5
The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens.
BMC Infect Dis. 2010 May 21;10:125. doi: 10.1186/1471-2334-10-125.
6
Detection of Typhoidal and Paratyphoidal Salmonella in Blood by Real-time Polymerase Chain Reaction.
Clin Infect Dis. 2015 Nov 1;61 Suppl 4(Suppl 4):S241-50. doi: 10.1093/cid/civ726.
7
Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections.
J Med Microbiol. 2007 Sep;56(Pt 9):1161-1166. doi: 10.1099/jmm.0.47197-0.
8
[Salmonella Typhi and typhoid fever, S. Paratyphi A and paratyphoid fever].
Nihon Rinsho. 2010 Jun;68 Suppl 6:197-200.
9
CRISPR is an optimal target for the design of specific PCR assays for salmonella enterica serotypes Typhi and Paratyphi A.
PLoS Negl Trop Dis. 2014 Jan 30;8(1):e2671. doi: 10.1371/journal.pntd.0002671. eCollection 2014.
10
Antimicrobial resistance, virulence profiles and molecular subtypes of Salmonella enterica serovars Typhi and Paratyphi A blood isolates from Kolkata, India during 2009-2013.
PLoS One. 2014 Aug 6;9(8):e101347. doi: 10.1371/journal.pone.0101347. eCollection 2014.
引用本文的文献
1
Rapid and specific differentiation of Salmonella enterica serotypes typhi and Paratyphi by multicolor melting curve analysis.
Gut Pathog. 2024 Aug 19;16(1):43. doi: 10.1186/s13099-024-00636-6.
2
Deletion of an immune evasion gene, , from a live serovar Typhimurium vaccine improves vaccine responses in aged mice.
Front Immunol. 2024 Jun 7;15:1376734. doi: 10.3389/fimmu.2024.1376734. eCollection 2024.
3
Deletion of serovar Typhi reduces bacterial adhesion and invasion toward host cells.
Front Microbiol. 2023 Nov 3;14:1301478. doi: 10.3389/fmicb.2023.1301478. eCollection 2023.
4
Novel Heptaplex PCR-Based Diagnostics for Enteric Fever Caused by Typhoidal Serovars and Its Applicability in Clinical Blood Culture.
J Microbiol Biotechnol. 2023 Nov 28;33(11):1457-1466. doi: 10.4014/jmb.2307.07031. Epub 2023 Aug 21.
5
Reduced immunogenicity of a live serovar Typhimurium vaccine in aged mice.
Front Immunol. 2023 May 3;14:1190339. doi: 10.3389/fimmu.2023.1190339. eCollection 2023.
6
The Prevalence of Undiagnosed Serovar in Healthy School-Aged Children in Osun State, Nigeria.
Pathogens. 2023 Apr 14;12(4):594. doi: 10.3390/pathogens12040594.
7
Prevalence of Salmonella in Stool During the Vaccine Impact on Diarrhea in Africa (VIDA) Study, 2015-2018.
Clin Infect Dis. 2023 Apr 19;76(76 Suppl1):S87-S96. doi: 10.1093/cid/ciac985.
8
Molecular Detection of and Antibiotic Susceptibility Evaluation of Isolated from Urinary Tract Infections.
Arch Razi Inst. 2022 Apr 30;77(2):573-578. doi: 10.22092/ARI.2022.357153.1985. eCollection 2022 Apr.
9
Multiplex PCR assay to detect high risk lineages of Salmonella Typhi and Paratyphi A.
PLoS One. 2022 Jul 22;17(7):e0267805. doi: 10.1371/journal.pone.0267805. eCollection 2022.
10
Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR.
Microbiol Spectr. 2022 Jun 29;10(3):e0024922. doi: 10.1128/spectrum.00249-22. Epub 2022 May 31.
本文引用的文献
1
Identification of diarrheagenic Escherichia coli isolated from infants and children in Dar es Salaam, Tanzania.
BMC Infect Dis. 2007 Aug 9;7:92. doi: 10.1186/1471-2334-7-92.
2
A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella Typhi.
J Microbiol Immunol Infect. 2007 Jun;40(3):222-6.
3
A novel linear plasmid mediates flagellar variation in Salmonella Typhi.
PLoS Pathog. 2007 May 11;3(5):e59. doi: 10.1371/journal.ppat.0030059.
4
High diversity of group A streptococcal emm types among healthy schoolchildren in Ethiopia.
Clin Infect Dis. 2006 May 15;42(10):1362-7. doi: 10.1086/503422. Epub 2006 Apr 13.
5
Burden of invasive disease caused by Haemophilus influenzae type b in Bamako, Mali: impetus for routine infant immunization with conjugate vaccine.
Pediatr Infect Dis J. 2005 Jun;24(6):533-7. doi: 10.1097/01.inf.0000164768.28135.0d.
6
Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA.
FEMS Microbiol Lett. 2005 Mar 1;244(1):199-205. doi: 10.1016/j.femsle.2005.01.039.
7
Detection and characterization of diarrheagenic Escherichia coli from young children in Hanoi, Vietnam.
J Clin Microbiol. 2005 Feb;43(2):755-60. doi: 10.1128/JCM.43.2.755-760.2005.
8
Bacteremia among children admitted to a rural hospital in Kenya.
N Engl J Med. 2005 Jan 6;352(1):39-47. doi: 10.1056/NEJMoa040275.
9
Invasive pneumococcal infections among hospitalized children in Bamako, Mali.
Pediatr Infect Dis J. 2004 Jul;23(7):642-9. doi: 10.1097/01.inf.0000130951.85974.79.
10
Cloning and characterization of the gene encoding the z66 antigen of Salmonella enterica serovar Typhi.
FEMS Microbiol Lett. 2004 May 15;234(2):239-46. doi: 10.1016/j.femsle.2004.03.030.
Zotero 插件