A simple method for isolating disomic strains of Saccharomyces cerevisiae.
作者信息
Zebrowski David C, Kaback David B
机构信息
Department of Microbiology and Molecular Genetics, UMDNJ-Graduate School of Biomedical Sciences, Newark, NJ 07101-1709, USA.
出版信息
Yeast. 2008 May;25(5):321-6. doi: 10.1002/yea.1590.
Abstract
A simple method to select disomic (N + 1) strains that should be applicable for almost any chromosome in Saccharomyces cerevisiae is presented. A diploid heterozygous for a KanMX knock-out mutation in an essential gene is sporulated and viable geneticin (G418)-resistant colonies selected. Disomic products of a missegregation or non-disjunction event containing a copy of both the wild-type essential gene and its complementary KanMX knock-out allele make up most of the viable colonies. This method has been used to isolate disomic haploids for a variety of chromosomes. It is appropriately named MARV (for missegregation-associated restoration of viability) and is easily adaptable to virtually any strain.
摘要
相似文献
1
A simple method for isolating disomic strains of Saccharomyces cerevisiae.
Yeast. 2008 May;25(5):321-6. doi: 10.1002/yea.1590.
2
Construction of PCR-ligated long flanking homology cassettes for use in the functional analysis of six unknown open reading frames from the left and right arms of Saccharomyces cerevisiae chromosome XV.
Yeast. 1998 Mar 15;14(4):391-9. doi: 10.1002/(SICI)1097-0061(19980315)14:4<391::AID-YEA235>3.0.CO;2-O.
3
Disruption and basic phenotypic analysis of six novel genes from the right arm of chromosome XII of Saccharomyces cerevisiae.
Yeast. 2001 Mar 30;18(5):473-80. doi: 10.1002/yea.704.
4
Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae.
Genetics. 1993 Jan;133(1):51-66. doi: 10.1093/genetics/133.1.51.
5
Haploidization in Saccharomyces cerevisiae induced by a deficiency in homologous recombination.
Genetics. 2012 May;191(1):279-84. doi: 10.1534/genetics.111.138180. Epub 2012 Feb 23.
6
Disruption of six Saccharomyces cerevisiae ORFs on chromosome XII results in three lethal disruptants.
Yeast. 2002 Jan 15;19(1):79-86. doi: 10.1002/yea.808.
7
Development of a system for multicopy gene integration in Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Jan;120:44-9. doi: 10.1016/j.mimet.2015.10.023. Epub 2015 Oct 31.
8
The mismatch repair system reduces meiotic homeologous recombination and stimulates recombination-dependent chromosome loss.
Mol Cell Biol. 1996 Nov;16(11):6110-20. doi: 10.1128/MCB.16.11.6110.
9
Deletion of six open reading frames from the left arm of chromosome IV of Saccharomyces cerevisiae.
Yeast. 1999 Sep 15;15(12):1275-85. doi: 10.1002/(SICI)1097-0061(19990915)15:12<1275::AID-YEA456>3.0.CO;2-4.
10
Disruption of six novel ORFs on the left arm of chromosome XII reveals one gene essential for vegetative growth of Saccharomyces cerevisiae.
Yeast. 1999 Sep 15;15(12):1287-96. doi: 10.1002/(SICI)1097-0061(19990915)15:12<1287::AID-YEA458>3.0.CO;2-S.
引用本文的文献
1
Genetically controlled mtDNA deletions prevent ROS damage by arresting oxidative phosphorylation.
Elife. 2022 Jul 8;11:e76095. doi: 10.7554/eLife.76095.
2
Stringent mating-type-regulated auxotrophy increases the accuracy of systematic genetic interaction screens with Saccharomyces cerevisiae mutant arrays.
Genetics. 2009 Jan;181(1):289-300. doi: 10.1534/genetics.108.092981. Epub 2008 Oct 28.
本文引用的文献
1
Effects of aneuploidy on cellular physiology and cell division in haploid yeast.
Science. 2007 Aug 17;317(5840):916-24. doi: 10.1126/science.1142210.
2
Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes.
Genetics. 1973 May;74(1):33-54. doi: 10.1093/genetics/74.1.33.
3
Synthetic genetic array analysis in Saccharomyces cerevisiae.
Methods Mol Biol. 2006;313:171-92. doi: 10.1385/1-59259-958-3:171.
4
The role of centromere alignment in meiosis I segregation of homologous chromosomes in Saccharomyces cerevisiae.
Genetics. 1999 Dec;153(4):1547-60. doi: 10.1093/genetics/153.4.1547.
5
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
Science. 1999 Aug 6;285(5429):901-6. doi: 10.1126/science.285.5429.901.
6
Rules of donor preference in saccharomyces mating-type gene switching revealed by a competition assay involving two types of recombination.
Genetics. 1997 Oct;147(2):399-407. doi: 10.1093/genetics/147.2.399.
7
Yeast spore germination: a requirement for Ras protein activity during re-entry into the cell cycle.
EMBO J. 1997 Oct 15;16(20):6171-81. doi: 10.1093/emboj/16.20.6171.
8
Meiotic nondisjunction and recombination of chromosome III and homologous fragments in Saccharomyces cerevisiae.
Genetics. 1993 Feb;133(2):149-58. doi: 10.1093/genetics/133.2.149.
9
Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae.
Genetics. 1993 Jan;133(1):51-66. doi: 10.1093/genetics/133.1.51.
10
Isolation and characterization of chromosome-gain and increase-in-ploidy mutants in yeast.
Genetics. 1993 Nov;135(3):677-91. doi: 10.1093/genetics/135.3.677.
Zotero 插件