水杨酸荧光猝灭法测定铁（III）

Determination of iron(III) with salicylic acid by the fluorescence quenching method.

作者信息

Cha K W, Park K W

机构信息

Department of Chemistry, Inha University, Incheon 402-751, South Korea.

出版信息

Talanta. 1998 Aug;46(6):1567-71. doi: 10.1016/s0039-9140(98)00032-0.

DOI:10.1016/s0039-9140(98)00032-0

PMID:18967288

Abstract

The spectrofluorimetric determination of Fe(3+) using salicylic acid as an emission reagent has been investigated by measuring the decrease of fluorescence intensity of salicylic acid due to the complexation of Fe(3+)-salicylic acid. An emission peak of salicylic acid, which is decreased linearly by addition of Fe(3+), occurs at 409 nm in aqueous solution with excitation at 299 nm. The determination of the ferric ion is in the range 1x10(-6)-10x10(-6) M Fe(3+) (0.0558-0.558 mug/ml) and the detection limit is 5x10(-8) M. The quenching effect of Fe(3+) on the fluorescence intensity of salicylic acid may be considered on the basis of complexation between salicylic acid and Fe(3+). The effects of foreign ions were investigated.

摘要

通过测量由于铁（Ⅲ）与水杨酸络合导致水杨酸荧光强度的降低，研究了以水杨酸为发射试剂荧光分光光度法测定铁（Ⅲ）。在299nm激发下，水杨酸在水溶液中的发射峰位于409nm处，加入铁（Ⅲ）后该峰强度呈线性下降。铁离子的测定范围为1×10⁻⁶ - 10×10⁻⁶ M Fe（Ⅲ）（0.0558 - 0.558μg/ml），检测限为5×10⁻⁸ M。铁（Ⅲ）对水杨酸荧光强度的猝灭作用可基于水杨酸与铁（Ⅲ）之间的络合来考虑。研究了外来离子的影响。