一种能选择性地使由亲水性离去基团活化的氨基酸带上电荷的柔性酶。

A flexizyme that selectively charges amino acids activated by a water-friendly leaving group.

作者信息

Niwa Nobuyoshi, Yamagishi Yusuke, Murakami Hiroshi, Suga Hiroaki

机构信息

Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.

出版信息

Bioorg Med Chem Lett. 2009 Jul 15;19(14):3892-4. doi: 10.1016/j.bmcl.2009.03.114. Epub 2009 Mar 28.

DOI:10.1016/j.bmcl.2009.03.114

PMID:19364647

Abstract

We have developed a new flexizyme (a flexible de novo tRNA acylation ribozyme) system, a pair of amino-derivatized benzyl thioester (ABT) and amino flexizyme (aFx). ABT bearing the ammonium ion was designed to render the acyl-donor substrates better water solubility. Although the previously reported flexizymes (eFx and dFx) did not show acylation activity for the ABT derivatives, a new flexizyme variant aFx, generated by in vitro selection against an amino acid activated ABT, exhibits high selectivity toward those activated ABT. The flexizymes system including aFx, eFx, and dFx enables us to prepare a wide variety of acyl-tRNAs charged with non-proteinogenic amino acids.

摘要

我们开发了一种新的柔性酶（一种灵活的从头合成tRNA酰化核酶）系统，一对氨基衍生化苄硫酯（ABT）和氨基柔性酶（aFx）。带有铵离子的ABT旨在使酰基供体底物具有更好的水溶性。尽管先前报道的柔性酶（eFx和dFx）对ABT衍生物没有显示出酰化活性，但通过针对氨基酸活化的ABT进行体外筛选产生的一种新的柔性酶变体aFx对那些活化的ABT表现出高选择性。包括aFx、eFx和dFx的柔性酶系统使我们能够制备多种携带非蛋白质氨基酸的酰基tRNA。