用于果蝇中转基因 RNAi 的全基因组规模 shRNA 资源。
A genome-scale shRNA resource for transgenic RNAi in Drosophila.
机构信息
Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA.
出版信息
Nat Methods. 2011 May;8(5):405-7. doi: 10.1038/nmeth.1592. Epub 2011 Apr 3.
Abstract
Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource.
摘要
现有的基于长双链发夹 RNA 的 Drosophila melanogaster 转基因 RNAi 资源是功能研究的有力工具，但在卵母细胞发生过程中基因敲低无效，卵母细胞发生是研究许多生物学问题的重要模型系统。我们表明，模仿内源性 microRNA 的 shRNA 在卵母细胞发生过程中非常有效地沉默基因表达。我们还描述了构建全基因组 shRNA 资源的进展。
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