蜡样芽孢杆菌属噬菌体BCP8-2（拟似巴士底狱样噬菌体群的新成员）的全基因组序列分析及推定金属β-内酰胺酶和SpoIIIE同源物的鉴定
Complete genome sequence analysis and identification of putative metallo-beta-lactamase and SpoIIIE homologs in Bacillus cereus group phage BCP8-2, a new member of the proposed Bastille-like group.
作者信息
Asare Paul Tetteh, Bandara Nadeeka, Jeong Tae-Yong, Ryu Sangryeol, Klumpp Jochen, Kim Kwang-Pyo
机构信息
Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Korea.
Department of Food and Animal Biotechnology, Center for Agricultural Biomaterials, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
出版信息
Arch Virol. 2015 Oct;160(10):2647-50. doi: 10.1007/s00705-015-2548-2. Epub 2015 Aug 4.
Bacillus cereus group-specific bacteriophage BCP8-2 exhibits a broad lysis spectrum among food and human isolates (330/364) of B. cereus while not infecting B. subtilis (50) or B. licheniformis (12) strains. Its genome is 159,071 bp long with 220 open reading frames, including genes for putative methyltransferases, metallo-beta-lactamase, and a sporulation-related SpoIIIE homolog, as wells as 18 tRNAs. Comparative genome analysis showed that BCP8-2 is related to the recently proposed Bastille-like phages, but not with either SPO1-like or Twort-like phages of the subfamily Spounavirinae.
蜡样芽孢杆菌属特异性噬菌体BCP8-2在蜡样芽孢杆菌的食品分离株和人类分离株（330/364）中表现出广泛的裂解谱，而不感染枯草芽孢杆菌（50株）或地衣芽孢杆菌（12株）菌株。其基因组长度为159,071 bp，有220个开放阅读框，包括假定的甲基转移酶、金属β-内酰胺酶和与芽孢形成相关的SpoIIIE同源物的基因，以及18个tRNA。比较基因组分析表明，BCP8-2与最近提出的类巴士底狱噬菌体相关，但与Spounavirinae亚科的SPO1样或Twort样噬菌体均无关联。
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