优化新一代基因组编辑工具。
Fine-Tuning Next-Generation Genome Editing Tools.
机构信息
Research and Innovation Centre, Genomics and Biology of Fruit Crop Department, Fondazione Edmund Mach (FEM), Via Mach 1, 38010 San Michele all'Adige (TN), Italy.
Department of Life Sciences and Systems Biology, Innovation Centre, University of Turin, Via Quarello 15/A, 10135 Turin, Italy.
出版信息
Trends Biotechnol. 2016 Jul;34(7):562-574. doi: 10.1016/j.tibtech.2016.03.007. Epub 2016 May 8.
The availability of genome sequences of numerous organisms and the revolution brought about by genome editing tools (e.g., ZFNs, TALENs, and CRISPR/Cas9 or RGENs) has provided a breakthrough in introducing targeted genetic changes both to explore emergent phenotypes and to introduce new functionalities. However, the wider application of these tools in biology, agriculture, medicine, and biotechnology is limited by off-target mutation effects. In this review, we compare available methods for detecting, measuring, and analyzing off-target mutations. Furthermore, we particularly focus on CRISPR/Cas9 regarding various methods, tweaks, and software tools available to nullify off-target effects.
大量生物体基因组序列的出现以及基因组编辑工具（如 ZFNs、TALENs 和 CRISPR/Cas9 或 RGENs）带来的革命，为引入靶向遗传变化提供了突破，无论是探索新兴表型还是引入新功能。然而，这些工具在生物学、农业、医学和生物技术中的更广泛应用受到脱靶突变效应的限制。在这篇综述中，我们比较了可用于检测、测量和分析脱靶突变的现有方法。此外，我们特别关注 CRISPR/Cas9，以及针对各种方法、调整和可用的软件工具来消除脱靶效应。
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