巴西南部地表水、沉积物及市售猪肉产品中的戊型肝炎病毒
Hepatitis E Virus in Surface Water, Sediments, and Pork Products Marketed in Southern Brazil.
作者信息
Heldt F H, Staggmeier R, Gularte J S, Demoliner M, Henzel A, Spilki F R
机构信息
Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Prédio Vermelho 2° Andar, Sala 205 RS 239, nº 2755, Novo Hamburgo, RS, Brazil.
出版信息
Food Environ Virol. 2016 Sep;8(3):200-5. doi: 10.1007/s12560-016-9243-7. Epub 2016 May 12.
Hepatitis E virus (HEV) is an emerging causative agent of food and waterborne hepatitis in human beings. HEV circulates among human populations and swine herds, and may be found in water contaminated by swine feces, as well as in pork. In the present study, 68 sediment samples and 250 water samples collected from the Sinos River tributaries, as well as 50 samples of pork products (pâté and blood sausage) marketed in the Sinos River watershed region, Brazil, were tested for the presence of HEV genome. Reverse-transcriptase polymerase chain reaction followed by nucleotide sequencing was used for detection and characterization of HEV genomes. Overall, 36 % of food samples tested positive for HEV (genotype 3). No sediment or water samples were positive. These results suggest that contaminated pork products may be a source of HEV infection within this region and indicate a need for better monitoring of food safety and swine herds.
戊型肝炎病毒（HEV）是一种新出现的可通过食物和水传播导致人类肝炎的病原体。HEV在人群和猪群中传播，可能存在于受猪粪便污染的水中以及猪肉中。在本研究中，对从巴西西诺斯河支流采集的68份沉积物样本和250份水样，以及在西诺斯河流域地区销售的50份猪肉制品（肉酱和血肠）样本进行了检测，以确定是否存在HEV基因组。采用逆转录聚合酶链反应及核苷酸测序来检测和鉴定HEV基因组。总体而言，36%的食品样本HEV检测呈阳性（基因型3）。沉积物或水样均未呈阳性。这些结果表明，受污染的猪肉制品可能是该地区HEV感染的一个来源，并表明需要更好地监测食品安全和猪群。
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