mRNA前体的可变聚腺苷酸化
Alternative polyadenylation of mRNA precursors.
作者信息
Tian Bin, Manley James L
机构信息
Department of Microbiology, Biochemistry and Molecular Genetics, New Jersey Medical School, Rutgers University, Newark, New Jersey 07103, USA.
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
出版信息
Nat Rev Mol Cell Biol. 2017 Jan;18(1):18-30. doi: 10.1038/nrm.2016.116. Epub 2016 Sep 28.
Abstract
Alternative polyadenylation (APA) is an RNA-processing mechanism that generates distinct 3' termini on mRNAs and other RNA polymerase II transcripts. It is widespread across all eukaryotic species and is recognized as a major mechanism of gene regulation. APA exhibits tissue specificity and is important for cell proliferation and differentiation. In this Review, we discuss the roles of APA in diverse cellular processes, including mRNA metabolism, protein diversification and protein localization, and more generally in gene regulation. We also discuss the molecular mechanisms underlying APA, such as variation in the concentration of core processing factors and RNA-binding proteins, as well as transcription-based regulation.
摘要
可变聚腺苷酸化（APA）是一种RNA加工机制，可在mRNA和其他RNA聚合酶II转录本上产生不同的3'末端。它在所有真核生物物种中广泛存在，被认为是基因调控的主要机制。APA具有组织特异性，对细胞增殖和分化很重要。在本综述中，我们讨论了APA在多种细胞过程中的作用，包括mRNA代谢、蛋白质多样化和蛋白质定位，以及更广泛的基因调控。我们还讨论了APA背后的分子机制，如核心加工因子和RNA结合蛋白浓度的变化，以及基于转录的调控。
相似文献
1
Alternative polyadenylation of mRNA precursors.
Nat Rev Mol Cell Biol. 2017 Jan;18(1):18-30. doi: 10.1038/nrm.2016.116. Epub 2016 Sep 28.
2
U1 snRNP telescripting: molecular mechanisms and beyond.
RNA Biol. 2021 Nov;18(11):1512-1523. doi: 10.1080/15476286.2021.1872963. Epub 2021 Jan 15.
3
Implications of polyadenylation in health and disease.
Nucleus. 2014;5(6):508-19. doi: 10.4161/nucl.36360. Epub 2014 Oct 31.
4
Alternative polyadenylation: methods, mechanism, function, and role in cancer.
J Exp Clin Cancer Res. 2021 Feb 1;40(1):51. doi: 10.1186/s13046-021-01852-7.
5
Alternative polyadenylation and RNA-binding proteins.
J Mol Endocrinol. 2016 Aug;57(2):F29-34. doi: 10.1530/JME-16-0070. Epub 2016 May 20.
6
The influence of 5' and 3' end structures on pre-mRNA metabolism.
J Cell Sci Suppl. 1995;19:13-9. doi: 10.1242/jcs.1995.supplement_19.2.
7
A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.
Mol Cell. 2019 Nov 21;76(4):590-599.e4. doi: 10.1016/j.molcel.2019.08.007. Epub 2019 Sep 12.
8
Global insights into alternative polyadenylation regulation.
RNA Biol. 2015;12(6):597-602. doi: 10.1080/15476286.2015.1040974.
9
The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export.
Mol Cell. 2019 Apr 4;74(1):118-131.e7. doi: 10.1016/j.molcel.2019.01.026. Epub 2019 Feb 25.
10
Systematic profiling of poly(A)+ transcripts modulated by core 3' end processing and splicing factors reveals regulatory rules of alternative cleavage and polyadenylation.
PLoS Genet. 2015 Apr 23;11(4):e1005166. doi: 10.1371/journal.pgen.1005166. eCollection 2015 Apr.
引用本文的文献
1
The role of the 3'-UTR of the chemokine receptor CCR2 and hnRNPA0 in regulating mRNA stability and subcellular distribution in human CD4 T cells.
Front Immunol. 2025 Aug 20;16:1655273. doi: 10.3389/fimmu.2025.1655273. eCollection 2025.
2
The CFII components PCF11 and Cbc change subnuclear localization as cells differentiate in an adult stem cell lineage.
MicroPubl Biol. 2025 Aug 15;2025. doi: 10.17912/micropub.biology.001718. eCollection 2025.
3
Integrating Full-Length and Second-Generation Transcriptomes to Elucidate the ApNPV-Induced Transcriptional Reprogramming in Midgut.
Insects. 2025 Jul 31;16(8):792. doi: 10.3390/insects16080792.
4
A cis-natural antisense RNA regulates alternative polyadenylation of SlSPX5 under Pi starvation in tomato.
Nat Commun. 2025 Aug 27;16(1):7981. doi: 10.1038/s41467-025-63406-1.
5
Computational methods for alternative polyadenylation and splicing in post-transcriptional gene regulation.
Exp Mol Med. 2025 Aug 14. doi: 10.1038/s12276-025-01496-z.
6
Regulation of transcriptome plasticity by mTOR signaling pathway.
Exp Mol Med. 2025 Aug 14. doi: 10.1038/s12276-025-01508-y.
7
Genome-wide CRISPR screen for human factors involved in alternative polyadenylation based on differential localization of CD47.
Sci Rep. 2025 Aug 10;15(1):29269. doi: 10.1038/s41598-025-14782-7.
8
The CFII components PCF11 and Cbc change subnuclear localization as cells differentiate in the male germ line adult stem cell lineage.
bioRxiv. 2025 Jul 22:2025.07.22.666077. doi: 10.1101/2025.07.22.666077.
9
Optogenetic Clustering of Human IRE1 Reveals Differential Regulation of Transcription and mRNA Splice Isoform Abundance by the UPR.
bioRxiv. 2025 Jul 21:2025.07.16.665212. doi: 10.1101/2025.07.16.665212.
10
Pre-mRNA processing factors differentially impact coordination between co-transcriptional cleavage and transcription termination.
Nat Commun. 2025 Aug 1;16(1):7086. doi: 10.1038/s41467-025-62555-7.
本文引用的文献
1
Intronic cleavage and polyadenylation regulates gene expression during DNA damage response through U1 snRNA.
Cell Discov. 2016 Jun 14;2:16013. doi: 10.1038/celldisc.2016.13. eCollection 2016.
2
UPF2-Dependent Nonsense-Mediated mRNA Decay Pathway Is Essential for Spermatogenesis by Selectively Eliminating Longer 3'UTR Transcripts.
PLoS Genet. 2016 May 5;12(5):e1005863. doi: 10.1371/journal.pgen.1005863. eCollection 2016 May.
3
Chromatoid Body Protein TDRD6 Supports Long 3' UTR Triggered Nonsense Mediated mRNA Decay.
PLoS Genet. 2016 May 5;12(5):e1005857. doi: 10.1371/journal.pgen.1005857. eCollection 2016 May.
4
Myriad Triple-Helix-Forming Structures in the Transposable Element RNAs of Plants and Fungi.
Cell Rep. 2016 May 10;15(6):1266-76. doi: 10.1016/j.celrep.2016.04.010. Epub 2016 Apr 28.
5
Role of miRNAs and alternative mRNA 3'-end cleavage and polyadenylation of their mRNA targets in cardiomyocyte hypertrophy.
Biochim Biophys Acta. 2016 May;1859(5):744-56. doi: 10.1016/j.bbagrm.2016.03.010. Epub 2016 Mar 24.
6
SR proteins are NXF1 adaptors that link alternative RNA processing to mRNA export.
Genes Dev. 2016 Mar 1;30(5):553-66. doi: 10.1101/gad.276477.115.
7
3'UTR Shortening Potentiates MicroRNA-Based Repression of Pro-differentiation Genes in Proliferating Human Cells.
PLoS Genet. 2016 Feb 23;12(2):e1005879. doi: 10.1371/journal.pgen.1005879. eCollection 2016 Feb.
8
Distal Alternative Last Exons Localize mRNAs to Neural Projections.
Mol Cell. 2016 Mar 17;61(6):821-33. doi: 10.1016/j.molcel.2016.01.020. Epub 2016 Feb 18.
9
Alternative cleavage and polyadenylation in spermatogenesis connects chromatin regulation with post-transcriptional control.
BMC Biol. 2016 Jan 22;14:6. doi: 10.1186/s12915-016-0229-6.
10
PAF Complex Plays Novel Subunit-Specific Roles in Alternative Cleavage and Polyadenylation.
PLoS Genet. 2016 Jan 14;12(1):e1005794. doi: 10.1371/journal.pgen.1005794. eCollection 2016 Jan.
Zotero 插件