小菜蛾（鳞翅目：菜蛾科）卵巢的转录组分析揭示了参与卵子发生的基因。

Transcriptome profiling of the Plutella xylostella (Lepidoptera: Plutellidae) ovary reveals genes involved in oogenesis.

作者信息

Peng Lu, Wang Lei, Yang Yi-Fan, Zou Ming-Min, He Wei-Yi, Wang Yue, Wang Qing, Vasseur Liette, You Min-Sheng

机构信息

State Key Laboratory of Ecological Pest Control for Fujian-Taiwan Crops and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Insect Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

出版信息

Gene. 2017 Dec 30;637:90-99. doi: 10.1016/j.gene.2017.09.020. Epub 2017 Sep 13.

DOI:10.1016/j.gene.2017.09.020

PMID:28916376

Abstract

BACKGROUND

As a specialized organ, the insect ovary performs valuable functions by ensuring fecundity and population survival. Oogenesis is the complex physiological process resulting in the production of mature eggs, which are involved in epigenetic programming, germ cell behavior, cell cycle regulation, etc. Identification of the genes involved in ovary development and oogenesis is critical to better understand the reproductive biology and screening for the potential molecular targets in Plutella xylostella, a worldwide destructive pest of economically major crops.

RESULTS

Based on transcriptome sequencing, a total of 7.88Gb clean nucleotides was obtained, with 19,934 genes and 1861 new transcripts being identified. Expression profiling indicated that 61.7% of the genes were expressed (FPKM≥1) in the P. xylostella ovary. GO annotation showed that the pathways of multicellular organism reproduction and multicellular organism reproduction process, as well as gamete generation and chorion were significantly enriched. Processes that were most likely relevant to reproduction included the spliceosome, ubiquitin mediated proteolysis, endocytosis, PI3K-Akt signaling pathway, insulin signaling pathway, cAMP signaling pathway, and focal adhesion were identified in the top 20 'highly represented' KEGG pathways. Functional genes involved in oogenesis were further analyzed and validated by qRT-PCR to show their potential predominant roles in P. xylostella reproduction.

CONCLUSIONS

Our newly developed P. xylostella ovary transcriptome provides an overview of the gene expression profiling in this specialized tissue and the functional gene network closely related to the ovary development and oogenesis. This is the first genome-wide transcriptome dataset of P. xylostella ovary that includes a subset of functionally activated genes. This global approach will be the basis for further studies on molecular mechanisms of P. xylostella reproduction aimed at screening potential molecular targets for integrated pest management.

摘要

背景

作为一个特殊的器官，昆虫卵巢通过确保繁殖力和种群生存发挥着重要作用。卵子发生是一个复杂的生理过程，其结果是产生成熟卵子，这些卵子参与表观遗传编程、生殖细胞行为、细胞周期调控等。鉴定参与卵巢发育和卵子发生的基因对于更好地理解生殖生物学以及筛选小菜蛾（一种对经济上重要作物具有全球破坏性的害虫）潜在的分子靶点至关重要。

结果

基于转录组测序，共获得7.88Gb的干净核苷酸，鉴定出19934个基因和1861个新转录本。表达谱分析表明，61.7%的基因在小菜蛾卵巢中表达（FPKM≥1）。基因本体（GO）注释显示，多细胞生物繁殖和多细胞生物繁殖过程以及配子生成和卵壳形成等途径显著富集。在排名前20的“高度富集”京都基因与基因组百科全书（KEGG）途径中，鉴定出了最可能与繁殖相关的过程，包括剪接体、泛素介导的蛋白质水解、内吞作用、PI3K-Akt信号通路、胰岛素信号通路、cAMP信号通路和粘着斑。通过qRT-PCR进一步分析和验证了参与卵子发生的功能基因，以显示它们在小菜蛾繁殖中潜在的主要作用。