Verification of and Gene and RNA Interference Combined with Irradiation to Mediate Fertility Changes in (Linnaeus).

(Linnaeus) mainly damages cruciferous crops and causes huge economic losses. Presently, chemical pesticides dominate its control, but prolonged use has led to the development of high resistance. In contrast, the sterile insect technique provides a preventive and control method to avoid the development of resistance. We discovered two genes related to the reproduction of and investigated the efficacy of combining irradiation with RNA interference for pest management. The results demonstrate that after injecting and , there was a significant decrease of 28.06% and 25.64% in egg production, and a decrease of 19.09% and 15.35% in the hatching rate compared to the control. The ratio of eupyrene sperm bundles to apyrene sperm bundles also decreased. and were identified as pivotal genes influencing male reproductive processes. We established a dose-response relationship for irradiation (0-200 Gy and 200-400 Gy) and derived the irradiation dose equivalent to RNA interference targeting and . Combining RNA interference with low-dose irradiation achieved a sub-sterile effect on , surpassing either irradiation or RNA interference alone. This study enhances our understanding of the genes associated with the reproduction of and proposes a novel approach for pest management by combining irradiation and RNA interference.