Yu Bingwei, Yan Shuangshuang, Zhou Huoyan, Dong Riyue, Lei Jianjun, Chen Changming, Cao Bihao
Department of Vegetable Science, College of Horticulture, South China Agricultural University, Guangzhou, China.
Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in South China, Ministry of Agriculture, Guangzhou, China.
Front Plant Sci. 2018 Jun 12;9:797. doi: 10.3389/fpls.2018.00797. eCollection 2018.
High temperature (HT) stress affects the growth and production of cucumbers, but genetic resources with high heat tolerance are very scarce in this crop. Calmodulin (CaM) has been confirmed to be related to the regulation of HT stress resistance in plants. , a CaM gene, was isolated from cucumber inbred line "02-8." Its expression was characterized in the present study. transcripts differed among the organs and tissues of cucumber plants and could be induced by HTs or abscisic acid, but not by salicylic acid. transcripts exhibited subcellular localization to the cytoplasm and nuclei of cells. Overexpression of in cucumber plants has the potential to improve their heat tolerance and protect against oxidative damage and photosynthesis system damage by regulating the expression of HT-responsive genes in plants, including chlorophyll catabolism-related genes under HT stress. Taken together, our results provide useful insights into stress tolerance in cucumber.
高温(HT)胁迫会影响黄瓜的生长和产量,但这种作物中具有高耐热性的遗传资源非常稀缺。钙调蛋白(CaM)已被证实与植物抗HT胁迫的调控有关。从黄瓜自交系“02-8”中分离出一个CaM基因,即CsCaM4。本研究对其表达特性进行了分析。CsCaM4转录本在黄瓜植株的器官和组织中存在差异,可被高温或脱落酸诱导,但不能被水杨酸诱导。CsCaM4转录本在细胞的细胞质和细胞核中表现出亚细胞定位。在黄瓜植株中过表达CsCaM4有可能通过调节植物中HT响应基因的表达来提高其耐热性,并防止氧化损伤和光合作用系统损伤,包括高温胁迫下与叶绿素分解代谢相关的基因。综上所述,我们的研究结果为黄瓜的胁迫耐受性提供了有用的见解。