hmiR-34c-3p 的上调通过靶向 EIF3D 抑制结肠癌细胞的增殖。

hmiR-34c-3p upregulation inhibits the proliferation of colon cancer cells by targeting EIF3D.

机构信息

Department of Gastrointestinal Surgery, Liaocheng People's Hospital, Shandong.

Department of Gastroenterology, Shandong Provincial Hospital, Jinan City, China.

出版信息

Anticancer Drugs. 2018 Nov;29(10):975-982. doi: 10.1097/CAD.0000000000000674.

DOI:10.1097/CAD.0000000000000674

PMID:30096129

Abstract

Our study desired to investigate how miR-34c-3p regulates colon cancer cell proliferation and what is the relationship between miR-34c-3p and EIF3D. HCEpiC (normal human colonic epithelial cells), SW620, HT-29, SW480, and HCT-116 (human colon cancer cells lines) were used in our study. SW620 cells were chosen and divided into blank, miR-34c-3p mimics, miR-34c-3p NC, miR-34c-3p inhibitors, Lv-EIF3D, Lv-NC, and miR-34c-3p mimics+Lv-EIF3D groups. qRT-PCR was used for the detection of miR-34c-3p and EIF3D mRNA expressions. Dual-luciferase reporter assay was performed to investigate the effect of miR-34c-3p on EIF3D. Western blot was performed to detect EIF3D, cyclin D1, and c-Myc expressions. Clone formation and MTT assay were used to measure cell proliferation ability. colon cancer cells had lower miR-34c-3p expression, but higher EIF3D expression compared with HCEpiC. EIF3D mRNA expression was regulated negatively by miR-34c-3p. In the miR-34c-3p mimics group, colon cancer cell proliferation was significantly decreased, whereas c-Myc and cyclin D1 expressions were downregulated. Colon cancer cell proliferation in miR-34c-3p inhibitors and Lv-EIF3D groups was enhanced, and c-Myc and cyclin D1 expressions were decreased. The results suggested that by targeting EIF3D, miR-34c-3p inhibited colon cancer cell proliferation.

摘要

我们的研究旨在探讨 miR-34c-3p 如何调节结肠癌细胞增殖，以及 miR-34c-3p 与 EIF3D 之间的关系。在我们的研究中使用了 HCEpiC（正常人类结肠上皮细胞）、SW620、HT-29、SW480 和 HCT-116（人结肠癌细胞系）。选择 SW620 细胞并将其分为空白组、miR-34c-3p 模拟物组、miR-34c-3p NC 组、miR-34c-3p 抑制剂组、Lv-EIF3D 组、Lv-NC 组和 miR-34c-3p 模拟物+Lv-EIF3D 组。使用 qRT-PCR 检测 miR-34c-3p 和 EIF3D mRNA 的表达。通过双荧光素酶报告基因实验研究 miR-34c-3p 对 EIF3D 的影响。通过 Western blot 检测 EIF3D、cyclin D1 和 c-Myc 的表达。通过克隆形成和 MTT 检测评估细胞增殖能力。与 HCEpiC 相比，结肠癌细胞中的 miR-34c-3p 表达水平较低，而 EIF3D 表达水平较高。EIF3D mRNA 的表达受 miR-34c-3p 的负调控。在 miR-34c-3p 模拟物组中，结肠癌细胞增殖明显减少，而 c-Myc 和 cyclin D1 的表达下调。miR-34c-3p 抑制剂和 Lv-EIF3D 组中结肠癌细胞增殖增强，c-Myc 和 cyclin D1 的表达下调。结果表明，miR-34c-3p 通过靶向 EIF3D 抑制结肠癌细胞增殖。