通过三维结构光照明显微镜原位探测细胞中大胞饮作用的特征及其内化物质的大小范围。
In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy.
机构信息
Institute of Frontier Medical Science, Jilin University, Changchun 130021, Jilin, People's Republic of China,
State Key Lab of Supramolecular Structure and Materials, Jilin University, Changchun 130021, Jilin, People's Republic of China.
出版信息
Int J Nanomedicine. 2018 Sep 12;13:5321-5333. doi: 10.2147/IJN.S171973. eCollection 2018.
BACKGROUND
Macropinocytosis can occur in various types of cells and displays multiple functions. However, real-time observation and characterization of the structures of macropinocytosis on the surface of the cell membrane is not yet possible.
MATERIALS AND METHODS
Here, we establish a real-time live cell surface imaging method using three-dimensional-structured illumination microscopy. Based on this, observation of the dynamic macropinocytosis process and morphological data of internalized structures on the surface of pancreatic cancer cells were achieved during macropinocytosis. Next, different-sized silica nanoparticles (SiO NPs) were used as the scale for identifying the size range of internalized substances of macropinocytosis in pancreatic cancer cells.
RESULTS AND CONCLUSION
Our study not only provides a practical method and more structural data for further investigation of macropinocytosis, but also makes deeper understanding of the cell response toward nanomaterials as well as nanodrugs possible.
背景
巨胞饮作用可发生于多种类型的细胞，具有多种功能。然而，对于细胞膜表面巨胞饮作用结构的实时观察和特征描述尚无法实现。
材料和方法
本研究建立了一种基于三维结构光照明显微镜的实时活细胞表面成像方法。在此基础上，我们在巨胞饮作用过程中对胰腺癌细胞表面的动态巨胞饮作用过程和内化结构的形态学数据进行了观察。接下来，我们使用不同大小的二氧化硅纳米颗粒（SiO NPs）作为标尺，以确定胰腺癌细胞巨胞饮作用内化物质的大小范围。
结果与结论
该研究不仅为进一步研究巨胞饮作用提供了一种实用的方法和更多的结构数据，还使人们能够更深入地了解细胞对纳米材料和纳米药物的反应。
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