一种基于罗丹明 6G-吡啶结合的新型荧光比色铜探针用于活细胞成像。
A novel fluoro-chromogenic Cu probe for living-cell imaging based on rhodamine 6G-pyridine conjugation.
机构信息
Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Ministry of Education) and Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin, 300387, China.
出版信息
Anal Bioanal Chem. 2019 May;411(14):3021-3028. doi: 10.1007/s00216-019-01748-8. Epub 2019 Mar 19.
A novel fluoro-chromogenic rhodamine spirolactam probe (RP) has been prepared through the condensation of rhodamine hydrazine and 2-acetylpyridine, which displayed the detection of Cu with high selectivity over a large number of other common metal ions. It shows a "turn-on" response to paramagnetic Cu with an about 12-fold enhancement, and a color change from colorless to red that is observable by the naked eye. These changes are ascribed to the ring-opening of the spirolactam in RP, and subsequent host-guest coordination. The 2:1 binding stoichiometry of RP to Cu was confirmed by Job's and B-H plots. The resulting fluorescence enhancement can be used to detect Cu at concentrations from 2.0 to 20.0 μM with a limit of detection of 0.21 μM, which was lower than the maximum allowable Cu level set by the WHO. Finally, RP has been utilized to monitor Cu in living cells and natural water. Graphical abstract.
一种新型的氟显色罗丹明螺环酰胺探针（RP）通过缩合罗丹明肼和 2-乙酰吡啶制备而成，该探针对 Cu 具有高选择性，可检测大量其他常见金属离子。它对顺磁性 Cu 表现出“开启”响应，增强约 12 倍，并且颜色从无色变为肉眼可见的红色。这些变化归因于 RP 中环的开环，以及随后的主体 - 客体配位。通过 Job 和 B-H 图证实了 RP 与 Cu 的 2:1 结合化学计量比。所得的荧光增强可用于检测浓度为 2.0 至 20.0 μM 的 Cu，检测限为 0.21 μM，低于世界卫生组织规定的最大允许 Cu 水平。最后，RP 已用于监测活细胞和天然水中的 Cu。
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