简单荧光开启型化学传感器用于选择性检测钡离子及其活细胞成像。
Simple Fluorescence Turn-On Chemosensor for Selective Detection of Ba Ion and Its Live Cell Imaging.
机构信息
Department of Life Science, Department of Energy Storage/Conversion Engineering of Graduate School, and Hydrogen and Fuel Cell Research Center , Chonbuk National University , Jeollabuk-do 54896 , Republic of Korea.
Department of Animal Biotechnology, College of Agriculture and Life Sciences , Chonbuk National University , Jeollabuk-do 54896 , Republic of Korea.
出版信息
Anal Chem. 2019 Aug 6;91(15):10095-10101. doi: 10.1021/acs.analchem.9b02057. Epub 2019 Jul 10.
A phenoxazine-based fluorescence chemosensor [(4-(-butyl)--(4-((4-((5-oxo-5-benzo[]phenoxazin-6-yl)amino)phenyl)sulfonyl)phenyl)benzamide)] was designed and synthesized by a simple synthetic methods. The fluorescence chemosensor selectively detects Ba in the existence of other alkaline metal ions. In addition, showed high selectivity and sensitivity for Ba detection. The detection limit of was 0.282 μM and the binding constant was 1.0 × 10 M in CHCN/HO (97.5:2.5 v/v, HEPES = 1.25 mM, pH 7.3) medium. This chemosensor functioned through the intramolecular charge transfer (ICT) mechanism, which was further confirmed by DFT studies. Live cell imaging in MCF-7 cells confirmed the cell permeability of and its capability for specific detection of Ba in living cells.
一种基于苯并吩嗪的荧光化学传感器 [(4-(-丁基)-[4-((4-((5-氧代-5-苯并[ ]吩嗪-6-基)氨基)苯基)磺酰基)苯基]苯甲酰胺)] 由简单的合成方法设计和合成。该荧光化学传感器选择性地在存在其他碱金属离子的情况下检测 Ba。此外，对 Ba 的检测表现出高选择性和灵敏度。在 CHCN/HO（97.5:2.5 v/v、HEPES = 1.25 mM、pH 7.3）介质中，的检测限为 0.282 μM，结合常数为 1.0 × 10 M。该化学传感器通过分子内电荷转移 (ICT) 机制起作用，这通过 DFT 研究得到了进一步证实。在 MCF-7 细胞中的活细胞成像证实了 的细胞通透性及其在活细胞中特异性检测 Ba 的能力。
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