一种用于检测活细胞内源性次氯酸盐的基于BODIPY的超快响应荧光探针。
An ultrafast responsive BODIPY-based fluorescent probe for the detection of endogenous hypochlorite in live cells.
作者信息
Qiao Liang, Nie Hailiang, Wu Yulong, Xin Fangyun, Gao Congcong, Jing Jing, Zhang Xiaoling
机构信息
Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.
出版信息
J Mater Chem B. 2017 Jan 21;5(3):525-530. doi: 10.1039/c6tb02774a. Epub 2016 Dec 20.
Hypochlorite plays a significant role in various physiological and pathological processes; however, its role is still less clear than the role of other reactive oxygen species. Herein, we report an ultrafast responsive (<0.2 s) and highly selective probe B-Ts for hypochlorite with high sensitivity and a detection limit as low as 7.5 nM. The probe was compatible in a wide pH range of 4-13. More importantly, experiments in live cells showed that the probe could penetrate the cell membrane easily and was capable of imaging endogenous and exogenous hypochlorite specifically with a fast response.
次氯酸盐在各种生理和病理过程中发挥着重要作用；然而，与其他活性氧物质相比，其次氯酸盐的作用仍不太明确。在此，我们报道了一种对次氯酸盐具有超快速响应（<0.2秒）、高选择性、高灵敏度且检测限低至7.5 nM的探针B-Ts。该探针在4-13的宽pH范围内具有兼容性。更重要的是，活细胞实验表明，该探针能够轻松穿透细胞膜，并且能够快速响应，特异性地对内源性和外源性次氯酸盐进行成像。
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