耐噬菌体鲍曼不动杆菌对抗菌药物重新敏感。
Bacteriophage-resistant Acinetobacter baumannii are resensitized to antimicrobials.
机构信息
School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia.
出版信息
Nat Microbiol. 2021 Feb;6(2):157-161. doi: 10.1038/s41564-020-00830-7. Epub 2021 Jan 11.
We characterized two bacteriophages, ΦFG02 and ΦCO01, against clinical isolates of Acinetobacter baumannii and established that the bacterial capsule is the receptor for these phages. Phage-resistant mutants harboured loss-of-function mutations in genes responsible for capsule biosynthesis, resulting in capsule loss and disruption of phage adsorption. The phage-resistant strains were resensitized to human complement, beta-lactam antibiotics and alternative phages and exhibited diminished fitness in vivo. Using a mouse model of A. baumannii infection, we showed that phage therapy was effective.
我们对两种针对鲍曼不动杆菌临床分离株的噬菌体（ΦFG02 和 ΦCO01）进行了特性分析，结果表明细菌荚膜是这些噬菌体的受体。噬菌体抗性突变株在负责荚膜生物合成的基因中存在功能丧失突变，导致荚膜丢失和噬菌体吸附中断。这些噬菌体抗性菌株对人补体、β-内酰胺类抗生素和替代噬菌体重新敏感，并在体内表现出降低的适应性。使用鲍曼不动杆菌感染的小鼠模型，我们表明噬菌体治疗是有效的。
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