LncRNA Promotes Clear Cell Renal Cell Carcinoma Progression by Targeting to Upregulate .

BACKGROUND

Long noncoding RNAs (lncRNAs) have been regarded as crucial regulators in many cancers, including clear cell renal cell carcinoma (ccRCC). This research aimed to explore the biological role and molecular mechanism of lncRNA in ccRCC.

MATERIALS AND METHODS

The expression levels of and were examined by RT-qPCR. Cell viability, migration and invasion were examined by CCK-8 and transwell assays. Luciferase reporter and RIP assays were adopted to verify the interaction between and or .

RESULTS

It was found that expression was highly expressed in ccRCC tissues and cells, and patients with high expression of had a short overall survival time. Moreover, depletion attenuated ccRCC cell viability, migration and invasion. In addition, was confirmed as a downstream target of and was inversely regulated by , and was a target of . Functional assays demonstrated that silencing or addition abolished the inhibitory effects of knockdown on ccRCC progression.

CONCLUSION

The results of the present study indicated that accelerated the development and progression of ccRCC by upregulating via sponging , suggesting a potential therapeutic target for patients with ccRCC.