Sadeq Omar, Mechchate Hamza, Es-Safi Imane, Bouhrim Mohamed, Jawhari Fatima Zahra, Ouassou Hayat, Kharchoufa Loubna, N AlZain Mashail, M Alzamel Nurah, Mohamed Al Kamaly Omkulthom, Bouyahya Abdelhakim, Benoutman Amina, Imtara Hamada
Faculty of Medicine, Arab American University Palestine, Jenin B.P. 240, Palestine.
Laboratory of Biotechnology, Environment, Agri-Food, and Health (LBEAS), Faculty of Sciences, University Sidi Mohamed Ben Abdellah (USMBA), Fez B.P. 1796, Morocco.
Plants (Basel). 2021 Apr 1;10(4):676. doi: 10.3390/plants10040676.
Pollen is a male flower gametophyte located in the anthers of stamens in angiosperms and a considerable source of compounds with health protective potential. In the present work, phytochemical screening was carried out as well as analysis of the antioxidant and antibacterial properties of pollen extracts from , and growing wild in Palestine. Phytochemical screening examined the total flavonol, flavone and phenolic content. The DPPH (1,2-Diphenyl-1-Picrylhydrazyl) and FRAP (ferric reducing power) methods were used to assess antioxidant propriety, and disc diffusion, minimum inhibitory and bactericidal concentration tests were used to test the pollen extract's antibacterial activity against multidrug-resistant (MDR) clinical isolates. The highest level of total phenolic was found in the extract of (56.78 ± 0.49 mg GAE (Gallic Acid Equivalent)/g). The flavone and flavonol content of samples ranged from 2.48 ± 0.05 to 8.03 ± 0.01 mg QE (Quercetin Equivalent)/g. pollen with IC50 values of 0.047 and 0.039 mg/mL in the DPPH and FRAP assays, respectively, showed the greatest radical scavenging action. In addition, this pollen showed a mild antibacterial action against the microorganisms studied, with MICs varying from 0.625 to 10 mg/mL and inhibition diameters ranging from 13.66 ± 1.5 to 16.33 ± 1.5 mm.
花粉是被子植物雄蕊花药中的雄花配子体,是具有健康保护潜力的化合物的重要来源。在本研究中,对巴勒斯坦野生生长的[植物名称1]、[植物名称2]和[植物名称3]的花粉提取物进行了植物化学筛选以及抗氧化和抗菌性能分析。植物化学筛选检测了总黄酮醇、黄酮和酚类含量。采用DPPH(1,2-二苯基-1-苦基肼)和FRAP(铁还原能力)方法评估抗氧化性能,采用纸片扩散法、最低抑菌浓度和杀菌浓度试验检测花粉提取物对多重耐药(MDR)临床分离株的抗菌活性。[植物名称1]提取物中的总酚含量最高(56.78±0.49毫克没食子酸当量(GAE)/克)。样品中的黄酮和黄酮醇含量范围为2.48±0.05至8.03±0.01毫克槲皮素当量(QE)/克。[植物名称3]花粉在DPPH和FRAP试验中的IC50值分别为0.047和0.0(此处原文有误,推测应为0.039)39毫克/毫升,显示出最强的自由基清除作用。此外,这种花粉对所研究的微生物表现出轻微的抗菌作用,MIC值在0.625至10毫克/毫升之间,抑菌直径在13.66±1.5至16.33±1.5毫米之间。