波兰的犬猫中分离到的 spp。
spp. in dogs and cats in Poland.
机构信息
Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Division of Parasitology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Poland.
出版信息
Ann Agric Environ Med. 2021 Jun 14;28(2):345-347. doi: 10.26444/aaem/120467. Epub 2020 Apr 29.
INTRODUCTION
spp. are common protozoan parasites of animals and humans. Due to their zoonotic potential it is important to know their species and prevalence in dogs and cats.
OBJECTIVE
The aim of the study was to determine the occurrence and molecular characteristics of spp. in dogs and cats in Poland.
MATERIAL AND METHODS
A total of 365 faecal samples (264 dogs and 101 cats) collected from animals living in Poland were analyzed using the Ziehl-Neelsen staining method and genus-specific PCR assay to amplify the 18S rRNA gene.
RESULTS
were found in 11 out of the 365 examined stool samples (3%). PCR analysis identified Cryptosporidium in 9 out of 264 canine stool samples (3.4%) and 2 out of 101 feline specimens (2%). DNA sequencing confirmed the presence of and in dogs and in cats.
CONCLUSIONS
This is the first molecular characterization of spp. infection in dogs and cats in Poland.
简介
spp. 是常见的动物和人类原生动物寄生虫。由于其具有人畜共患的潜力，因此了解其在狗和猫中的种类和流行情况非常重要。
目的
本研究旨在确定波兰狗和猫中 spp. 的发生情况和分子特征。
材料和方法
共分析了来自波兰的 365 份粪便样本（264 只狗和 101 只猫），使用齐尔-尼尔森染色法和种特异性 PCR 检测扩增 18S rRNA 基因。
结果
在 365 个检查的粪便样本中，有 11 个（3%）发现了 。PCR 分析在 264 只犬粪便样本中的 9 只（3.4%）和 101 只猫粪便样本中的 2 只（2%）中鉴定出了隐孢子虫。DNA 测序证实了犬中存在 和 ，以及猫中存在 。
结论
这是波兰首次对狗和猫中 spp. 感染进行的分子特征描述。
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