姜黄素和姜酮通过 D-半乳糖诱导的大鼠脑和心脏衰老相关氧化改变。
Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats' Brain and Heart.
机构信息
Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.
Department of Histology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
出版信息
Int J Mol Sci. 2021 Jun 25;22(13):6839. doi: 10.3390/ijms22136839.
D-galactose (D-gal) administration causes oxidative disorder and is widely utilized in aging animal models. Therefore, we subcutaneously injected D-gal at 200 mg/kg BW dose to assess the potential preventive effect of thymoquinone (TQ) and curcumin (Cur) against the oxidative alterations induced by D-gal. Other than the control, vehicle, and D-gal groups, the TQ and Cur treated groups were orally supplemented at 20 mg/kg BW of each alone or combined. TQ and Cur effectively suppressed the oxidative alterations induced by D-gal in brain and heart tissues. The TQ and Cur combination significantly decreased the elevated necrosis in the brain and heart by D-gal. It significantly reduced brain caspase 3, calbindin, and calcium-binding adapter molecule 1 (IBA1), heart caspase 3, and BCL2. Expression of mRNA of the brain and heart , , , and were significantly downregulated in the TQ and Cur combination group along with upregulation of in comparison with the D-gal group. Data suggested that the TQ and Cur combination is a promising approach in aging prevention.
半乳糖（D-gal）给药会导致氧化紊乱，并且广泛用于衰老动物模型。因此，我们以 200mg/kgBW 的剂量皮下注射 D-gal，以评估胸腺醌（TQ）和姜黄素（Cur）对 D-gal 诱导的氧化改变的潜在预防作用。除了对照组、载体组和 D-gal 组之外，TQ 和 Cur 处理组分别以 20mg/kgBW 的剂量单独或联合口服补充。TQ 和 Cur 有效抑制了 D-gal 在脑组织和心脏组织中引起的氧化改变。TQ 和 Cur 的联合使用显著降低了 D-gal 引起的大脑和心脏的坏死。它还显著降低了大脑中的半胱氨酸天冬氨酸蛋白酶 3（caspase 3）、钙结合蛋白（calbindin）和钙结合接头蛋白 1（IBA1）以及心脏中的 caspase 3 和 BCL2 的表达。与 D-gal 组相比，TQ 和 Cur 联合组大脑和心脏中 、 、 、 和 的 mRNA 表达显著下调，而 的表达上调。数据表明，TQ 和 Cur 的联合使用是一种有前途的抗衰老方法。
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