长链非编码RNA AC007255.1，一种食管癌中与免疫相关的预后增强子RNA。
LncRNA AC007255.1, an immune-related prognostic enhancer RNA in esophageal cancer.
作者信息
Wang Qingqing, Yu Xiaoyan, Yang Ningning, Xu Lu, Zhou Yunfeng
机构信息
Department of Radiation Oncology and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, China.
Hubei Cancer Clinical Study Center, Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital, Wuhan University, Wuhan, China.
出版信息
PeerJ. 2021 Jul 14;9:e11698. doi: 10.7717/peerj.11698. eCollection 2021.
BACKGROUND
Growing evidence has suggested that enhancer RNAs (eRNAs), a set of long non-coding RNAs (lncRNAs) that were derived from active enhancer regions, play critical roles in regulating gene expression in human cancers. Nevertheless potential functions of eRNAs in esophageal cancer ESCA have not yet been expounded. Here, this study aimed to explore key prognostic eRNAs in ESCA.
METHODS
LncRNAs that were transcribed from active enhancer regions were analyzed utilizing the PreSTIGE algorithm, followed by prediction of their target genes. Based on the ESCA RNA-seq data from the TANRIC database, overall survival (OS)-related eRNAs were determined. The correlation between AC007255.1 expression and various clinical traits of ESCA was calculated. Functional enrichment analysis was presented based on its co-expressed genes. Based on the TIMER database, we analyzed correlations between AC007255.1 expression and immune infiltration levels. qRT-PCR was utilized to validate the expression of AC007255.1 and PRR15 in ESCA and normal tissues.
RESULTS
Totally, 2,695 lncRNAs were transcribed from active enhancer regions. Among them, 33 were significantly related to OS. AC007255.1 was a key eRNA. PRR15 was a target gene of AC007255.1 (correlation coefficient = 0.936). Patients with high AC007255.1 expression indicated poor OS time. There were significant correlations between AC007255.1 expression and clinical characteristics like pathological TNM, grade and stage. AC007255.1 was closely related to tight junction and neutrophil activation involved in immune response. Moreover, AC007255.1 expression was related to the infiltration levels of B cell, dendritic cell and neutrophil. qRT-PCR results confirmed that AC007255.1 and PRR15 were both up-regulated in ESCA tissues, and there was a positive correlation between the two.
CONCLUSION
Our findings identified a novel immune-related eRNA AC007255.1 in ESCA, which could be a promising prognostic factor for ESCA.
背景
越来越多的证据表明，增强子RNA（eRNA）是一类源自活性增强子区域的长链非编码RNA（lncRNA），在调控人类癌症基因表达中发挥关键作用。然而，eRNA在食管癌（ESCA）中的潜在功能尚未阐明。本研究旨在探索ESCA中关键的预后eRNA。
方法
利用PreSTIGE算法分析从活性增强子区域转录的lncRNA，随后预测其靶基因。基于来自TANRIC数据库的ESCA RNA测序数据，确定与总生存期（OS）相关的eRNA。计算AC007255.1表达与ESCA各种临床特征之间的相关性。基于其共表达基因进行功能富集分析。基于TIMER数据库，分析AC007255.1表达与免疫浸润水平之间的相关性。采用qRT-PCR验证AC007255.1和PRR15在ESCA组织和正常组织中的表达。
结果
总共从活性增强子区域转录出2695个lncRNA。其中，33个与OS显著相关。AC007255.1是关键的eRNA。PRR15是AC007255.1的靶基因（相关系数=0.936）。AC007255.1高表达的患者OS时间较差。AC007255.1表达与病理TNM、分级和分期等临床特征之间存在显著相关性。AC007255.1与参与免疫反应的紧密连接和中性粒细胞活化密切相关。此外，AC007255.1表达与B细胞、树突状细胞和中性粒细胞的浸润水平相关。qRT-PCR结果证实AC007255.1和PRR15在ESCA组织中均上调，且两者呈正相关。
结论
我们的研究发现了ESCA中一种新的免疫相关eRNA AC007255.1，它可能是ESCA一个有前景的预后因素。
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