使用 Ga-NOTA-c(NGR) 对手术大鼠模型中缺血/再灌注介导的氨肽酶 N 表达进行成像。
Imaging of Ischemia/Reperfusion-mediated Aminopeptidase N Expression in Surgical Rat Model Using Ga-NOTA-c(NGR).
机构信息
Division of Nuclear Medicine and Translational Imaging, Department of Medical Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Gyula Petrányi Doctoral School of Allergy and Clinical Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
出版信息
In Vivo. 2022 Mar-Apr;36(2):657-666. doi: 10.21873/invivo.12750.
BACKGROUND/AIM: Previous studies have already shown that Gallium(Ga)-labeled NGR-based radiopharmaceuticals specifically bind to the neoangiogenic molecule Aminopeptidase N (APN/CD13). The aim of this study was to evaluate the applicability of Ga-NOTA-c(NGR) in the in vivo detection of the temporal changes of APN/CD13 expression in the diabetic retinopathy rat model using positron emission tomography (PET).
MATERIALS AND METHODS
Ischemia/reperfusion injury was initiated by surgical ligation of the left bulbus oculi of rats. In vivo PET imaging studies were performed after the surgery using Ga-NOTA-c(NGR).
RESULTS
Significantly higher Ga-NOTA-c(NGR) uptake was observed in the surgically-ligated left bulbus, compared to the bulbus of the non-surgical group at each investigated time point. The western blot and histological analysis confirmed the increased expression of the neo-angiogenic marker APN/CD13.
CONCLUSION
Ga-NOTA-c(NGR) is a suitable radiotracer for the detection of the temporal changes of the ischemia/reperfusion-mediated expression of APN/CD13 in the surgically induced diabetic retinopathy rat model.
背景/目的：之前的研究已经表明，镓（Ga）标记的基于 NGR 的放射性药物特异性结合新生血管生成分子氨基肽酶 N（APN/CD13）。本研究旨在评估 Ga-NOTA-c（NGR）在使用正电子发射断层扫描（PET）检测糖尿病视网膜病变大鼠模型中 APN/CD13 表达的时间变化中的适用性。
材料和方法
通过手术结扎大鼠左眼球来引发缺血/再灌注损伤。手术后使用 Ga-NOTA-c（NGR）进行体内 PET 成像研究。
结果
与非手术组相比，在每个研究时间点，手术结扎的左眼球中观察到 Ga-NOTA-c（NGR）的摄取明显更高。Western blot 和组织学分析证实了新生血管标志物 APN/CD13 的表达增加。
结论
Ga-NOTA-c（NGR）是一种合适的示踪剂，可用于检测手术诱导的糖尿病视网膜病变大鼠模型中缺血/再灌注介导的 APN/CD13 表达的时间变化。
Zotero 插件