遗传密码扩展中的“非流行”正交对。
"Not-so-popular" orthogonal pairs in genetic code expansion.
机构信息
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, USA.
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, Arkansas, USA.
出版信息
Protein Sci. 2023 Feb;32(2):e4559. doi: 10.1002/pro.4559.
During the past decade, genetic code expansion has been proved to be a powerful tool for protein studies and engineering. As the key part, a series of orthogonal pairs have been developed to site-specifically incorporate hundreds of noncanonical amino acids (ncAAs) into proteins by using bacteria, yeast, mammalian cells, animals, or plants as hosts. Among them, the pair of tyrosyl-tRNA synthetase/tRNA from Methanococcus jannaschii and the pair of pyrrolysyl-tRNA synthetase/tRNA from Methanosarcina species are the most popular ones. Recently, other "not-so-popular" orthogonal pairs have started to attract attentions, because they can provide more choices of ncAA candidates and are necessary for simultaneous incorporation of multiple ncAAs into a single protein. Here, we summarize the development and applications of those "not-so-popular" orthogonal pairs, providing guidance for studying and engineering proteins.
在过去的十年中，遗传密码扩展已被证明是研究和工程蛋白质的强大工具。作为关键部分，已经开发出一系列正交对，通过使用细菌、酵母、哺乳动物细胞、动物或植物作为宿主，将数百种非天然氨基酸（ncAA）定点掺入蛋白质中。其中，来源于产甲烷球菌（Methanococcus jannaschii）的酪氨酸 tRNA 合成酶/tRNA 对和来源于甲烷八叠球菌（Methanosarcina species）的吡咯赖氨酸 tRNA 合成酶/tRNA 对是最受欢迎的一对。最近，其他“不太受欢迎”的正交对开始引起关注，因为它们可以提供更多 ncAA 候选物的选择，并且对于将多个 ncAA 同时掺入单个蛋白质是必需的。在这里，我们总结了这些“不太受欢迎”的正交对的发展和应用，为研究和工程蛋白质提供了指导。
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