一种简单、经济高效、高通量的深血浆蛋白质组学耗竭策略。
A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics.
机构信息
Department of Pathology, Boston Children's Hospital, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
出版信息
Sci Adv. 2023 Mar 29;9(13):eadf9717. doi: 10.1126/sciadv.adf9717.
Abstract
We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample.
摘要
我们介绍了一种经济高效、稳健的高通量兼容血浆耗竭方法，可深度分析血浆，每次运行可检测超过 1300 种蛋白质，每天通量为 60 个样本。该方法已经通过处理超过 3000 个样本得到了充分验证，在耗竭步骤的成本约为每个样本 2.5 美元，没有明显的批次效应。
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