Intratumoral CXCL13 CD160 CD8 T cells promote the formation of tertiary lymphoid structures to enhance the efficacy of immunotherapy in advanced gastric cancer.

BACKGROUND

Stage IV gastric cancer is a highly heterogeneous and lethal tumor with few therapeutic strategies. The combination of programmed cell death protein 1 inhibitors and chemotherapy is currently the standard frontline treatment regimen for advanced gastric cancer. Nevertheless, it remains a great challenge to screen the beneficiaries of immunochemotherapy and expand indications for this treatment regimen.

METHODS

We conducted a pathological assessment to ascertain the importance of tertiary lymphoid structures based on the tissue samples collected from patients with stage IV gastric cancer (n=15) both prior to and following immunochemotherapy treatment. Additionally, we used spatial (n=10) and single-cell transcriptional analysis (n=97) to investigate the key regulators of tertiary lymphoid structures (TLSs). Multiplex immunofluorescence and image analysis (n=34) were performed to explore the association between tumor-infiltrating CXCL13 CD160 CD8 T cells and TLSs. The relationship between CXCL13 CD160 CD8 T cells and the responsiveness to immunotherapy was also evaluated by multiplex immunofluorescence and image analysis approaches (n=15). Furthermore, we explored the intrinsic characteristics of CXCL13 CD160 CD8 T cells through various experimental techniques, including quantitative reverse transcription-PCR, western blot, and flow cytometry.

RESULTS

We found that responders exhibited higher levels of TLSs and CXCL13 CD160 CD8 T cells in biopsy tissues prior to immunochemotherapy compared with non-responders. Following conversion therapy, responders also had a higher percentage of mature TLSs and a higher number of CXCL13 CD160 CD8 T cells in surgical resections. Moreover, we discovered that vitamin B in CD160 CD8 T cells could reduce the ubiquitination modification of HIF-1α by MDM2, thereby attenuating the degradation of HIF-1α. Consequently, this led to the transcriptional upregulation of CXCL13 expression, facilitating the recruitment of CXCR5 B cells and the formation of TLSs.

CONCLUSION

The number and maturity of TLSs, along with the extent of CXCL13 CD160 CD8 T-cell infiltration, might function as potential indicators for assessing the effectiveness of immunotherapy in treating gastric malignancies. Furthermore, our research suggests that vitamin B could enhance the secretion of CXCL13 by CD160 CD8 T cells by reducing the degradation of HIF-1α. Additionally, we demonstrate that vitamin B supplementation or targeting pyridoxal kinase could substantially improve the efficacy of immunotherapies for gastric cancer.