来自囊性纤维化患者的烟曲霉狭义种的遗传多样性。

Aspergillus fumigatus sensu stricto genetic diversity from cystic fibrosis patients.

作者信息

Melo Aryse Martins, Poester Vanice Rodrigues, Trápaga Mariana Rodrigues, Faria Fernando Azevedo, Aquino Valério, Severo Cecília Bittencourt, Stevens David A, Veríssimo Cristina, Sabino Raquel, Xavier Melissa Orzechowski

机构信息

One Health Disease Control Lab Group, Department of Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.

出版信息

Int J Med Microbiol. 2024 Dec;317:151639. doi: 10.1016/j.ijmm.2024.151639. Epub 2024 Oct 22.

DOI:10.1016/j.ijmm.2024.151639

PMID:39490213

Abstract

We aimed to access the genetic diversity of Apergillus fumigatus strains obtained from cystic fibrosis (CF) patients from southern Brazil. A. fumigatus sensu stricto isolates from respiratory clinical specimens were genotyped by microsatellite markers and azole resistance was evaluated by azole-agar screening. Twenty-seven isolates from twenty-seven patients showed a high genetic diversity, with the differentiation of 25 different genotypes (25 unique and one common to two isolates). All isolates were susceptible to the azoles tested. We believe that prospectively monitoring A. fumigatus genetic diversity is essential to identify interpatient transmission and outbreaks, as is the identification of resistant strains.

摘要

我们旨在研究从巴西南部囊性纤维化（CF）患者中分离出的烟曲霉菌株的遗传多样性。通过微卫星标记对呼吸道临床标本中的狭义烟曲霉分离株进行基因分型，并通过唑类琼脂筛选评估唑类耐药性。来自27名患者的27株分离株显示出高度的遗传多样性，分化出25种不同的基因型（25种独特基因型以及两种分离株共有的一种基因型）。所有分离株对所测试的唑类均敏感。我们认为，前瞻性监测烟曲霉的遗传多样性对于识别患者间传播和暴发至关重要，识别耐药菌株也是如此。