一种无泵无管微流控装置可实现……的体外长期发育 。 （原文句子不完整）
A Pumpless and Tubeless Microfluidic Device Enables Extended In Vitro Development of .
作者信息
Gunasekera Samantha, Thierry Benjamin, Cheah Edward, King Brendon, Monis Paul, Carr Jillian M, Chopra Abha, Watson Mark, O'Dea Mark, Ryan Una
机构信息
Harry Butler Institute, College of Environmental and Life Sciences, Murdoch University, Murdoch, Western Australia, Australia.
Future Industries Institute, University of South Australia, Adelaide, South Australia, Australia.
出版信息
Open Forum Infect Dis. 2024 Oct 16;11(11):ofae625. doi: 10.1093/ofid/ofae625. eCollection 2024 Nov.
BACKGROUND
The enteric parasite remains a treatment challenge for drinking water utilities globally due to its resistance to chlorine disinfection. However, the lack of an in vitro culture system for that is both cost-effective and reliable remains a key bottleneck in research.
METHODS
Here we report that the microfluidic culture of human ileocecal colorectal adenocarcinoma (HCT-8) cells under fluid shear stress enables the extended development of . Specifically, the growth of in a user-friendly pumpless microfluidic device was assessed using immunofluorescence assays, scanning electron microscopy, and quantitative polymerase chain reaction, which revealed that development continued for 10 days in total.
RESULTS
Oocysts produced within the microfluidic device were infective to fresh HCT-8 monolayers; however, these oocysts were only present at low levels.
CONCLUSIONS
We anticipate that such microfluidic approaches will facilitate a wide range of in vitro studies on and may have the potential to be further developed as a routine infectivity assessment tool for the water industry.
背景
由于对氯消毒具有抗性，肠道寄生虫仍然是全球饮用水处理行业面临的治疗挑战。然而，缺乏一种既经济高效又可靠的体外培养系统仍然是该研究的关键瓶颈。
方法
在此我们报告，在流体剪切应力下对人回盲部结肠直肠腺癌（HCT-8）细胞进行微流控培养能够使该寄生虫得到长期发育。具体而言，使用免疫荧光测定、扫描电子显微镜和定量聚合酶链反应评估了该寄生虫在用户友好的无泵微流控装置中的生长情况，结果显示其发育总共持续了10天。
结果
微流控装置内产生的卵囊对新鲜的HCT-8单层细胞具有感染性；然而，这些卵囊仅以低水平存在。
结论
我们预计，这种微流控方法将促进对该寄生虫的广泛体外研究，并且可能有潜力进一步开发成为水行业的常规感染性评估工具。
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